Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics
文献类型：期刊论文
| 作者 | Li, Tao1; Li, Chulin1; Wang, Weinan1; Wu, Hualian1; Wu, Houbo1; Xu, Jin2; Xiang, Wenzhou1 |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2024-02-01 |
| 卷号 | 22期号:2页码:17 |
| 关键词 | Porphyridium EPA lipidomics omega 3 and omega 6 pathway fatty acid desaturase |
| DOI | 10.3390/md22020082 |
| 通讯作者 | Xu, Jin(xujin@ms.giec.ac.cn) ; Xiang, Wenzhou(xwz@scsio.ac.cn) |
| 英文摘要 | The marine red microalga Porphyridium can simultaneously synthesize long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (C20:5, EPA) and arachidonic acid (C20:4, ARA). However, the distribution and synthesis pathways of EPA and ARA in Porphyridium are not clearly understood. In this study, Porphyridium cruentum CCALA 415 was cultured in nitrogen-replete and nitrogen-limited conditions. Fatty acid content determination, transcriptomic, and lipidomic analyses were used to investigate the synthesis of ARA and EPA. The results show that membrane lipids were the main components of lipids, while storage lipids were present in a small proportion in CCALA 415. Nitrogen limitation enhanced the synthesis of storage lipids and omega 6 fatty acids while inhibiting the synthesis of membrane lipids and omega 3 fatty acids. A total of 217 glycerolipid molecular species were identified, and the most abundant species included monogalactosyldiglyceride (C16:0/C20:5) (MGDG) and phosphatidylcholine (C16:0/C20:4) (PC). ARA was mainly distributed in PC, and EPA was mainly distributed in MGDG. Among all the fatty acid desaturases (FADs), the expressions of Delta 5FAD, Delta 6FAD, Delta 9FAD, and Delta 12FAD were up-regulated, whereas those of Delta 15FAD and Delta 17FAD were down-regulated. Based on these results, only a small proportion of EPA was synthesized through the omega 3 pathway, while the majority of EPA was synthesized through the omega 6 pathway. ARA synthesized in the ER was likely shuttled into the chloroplast by DAG and was converted into EPA by Delta 17FAD. |
| WOS关键词 | FATTY-ACIDS ; EICOSAPENTAENOIC ACID ; NITROGEN STARVATION ; GROWTH ; BIOSYNTHESIS ; IDENTIFICATION ; DESATURASES ; METABOLISM ; LIPIDS |
| 资助项目 | Guangdong Basic and applied Basic Research Foundation |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001175017800001 |
| 出版者 | MDPI |
| 资助机构 | Guangdong Basic and applied Basic Research Foundation |
| 源URL | [http://ir.giec.ac.cn/handle/344007/41072]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Xu, Jin; Xiang, Wenzhou |
| 作者单位 | 1.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med,CAS Key Lab Trop, Guangzhou 510301, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Tao,Li, Chulin,Wang, Weinan,et al. Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics[J]. MARINE DRUGS,2024,22(2):17. |
| APA | Li, Tao.,Li, Chulin.,Wang, Weinan.,Wu, Hualian.,Wu, Houbo.,...&Xiang, Wenzhou.(2024).Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics.MARINE DRUGS,22(2),17. |
| MLA | Li, Tao,et al."Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Porphyridium cruentum Using Lipidomics and Transcriptomics".MARINE DRUGS 22.2(2024):17. |
入库方式： OAI收割
来源：广州能源研究所
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