Dual-Pathway Lateral Flow Assay for Rapid and Sensitive SARS-CoV-2 RNA Detection via CRISPR/Cas13a-Mediated SERS
文献类型：期刊论文
| 作者 | Joung, Younju4; Han, Do Kyoung3; Jang, Hyowon2; Kang, Taejoon2,5; Chen, Lingxin1; Choo, Jaebum4 |
| 刊名 | ACS SENSORS
 |
| 出版日期 | 2025-08-22 |
| 卷号 | 10期号:8页码:6253-6262 |
| 关键词 | surface-enhanced Raman scattering lateral flow assaystrips dual-pathway CRISPR/Cas13a SARS-CoV-2 |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.5c02084 |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr) |
| 英文摘要 | Reverse transcription-polymerase chain reaction (RT-PCR) has been the gold standard for SARS-CoV-2 detection during the COVID-19 pandemic. However, its requirement for RNA-to-DNA conversion, reliance on centralized laboratory infrastructure, and lengthy turnaround times have limited its application in point-of-care (POC) settings. CRISPR/Cas13a-mediated lateral flow assays (LFAs) have emerged as promising alternatives for direct RNA analysis, yet their two-step workflows introduce procedural complexity and reduce sensitivity. To overcome these limitations, we developed a dual-pathway LFA strip based on surface-enhanced Raman scattering (SERS), which integrates CRISPR/Cas13a-mediated RNA cleavage and SERS detection into a single, portable platform. The device utilizes five vertically stacked paper layers with distinct geometries, enabling sequential CRISPR reaction and SERS quantification through two independent pathways. When tested with SARS-CoV-2 ORF1ab RNA targets, the system exhibited an 80-fold increase in sensitivity and a 10 min reduction in assay time compared to conventional fluorescence assays. Clinical validation using 18 samples (13 positives and 5 negatives) demonstrated high diagnostic accuracy, fully consistent with RT-PCR results. By unifying CRISPR-based RNA recognition and SERS signal amplification in a user-friendly format, this dual-pathway LFA strip offers a rapid, ultrasensitive, and practical diagnostic tool for infectious diseases in POC settings. |
| WOS关键词 | AMPLIFICATION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001559994000001 |
| 资助机构 | National Natural Science Foundation of China ; National Research Foundation of Korea ; Technology Innovation Program ; Ministry of Trade, Industry, and Energy (MOTIE, Korea) ; National Nature Science Foundation of China ; Korea Basic Science Institute ; KRIBB Research Initiative Program |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40796]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, Lingxin; Choo, Jaebum |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Korea Res Inst Biosci & Biotechnol, Bionanotechnol Res Ctr, Daejeon 34141, South Korea 3.Korea Basic Sci Inst, Div Mat Anal & Res, Daejeon 34133, South Korea 4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea 5.Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea |
| 推荐引用方式 GB/T 7714 | Joung, Younju,Han, Do Kyoung,Jang, Hyowon,et al. Dual-Pathway Lateral Flow Assay for Rapid and Sensitive SARS-CoV-2 RNA Detection via CRISPR/Cas13a-Mediated SERS[J]. ACS SENSORS,2025,10(8):6253-6262. |
| APA | Joung, Younju,Han, Do Kyoung,Jang, Hyowon,Kang, Taejoon,Chen, Lingxin,&Choo, Jaebum.(2025).Dual-Pathway Lateral Flow Assay for Rapid and Sensitive SARS-CoV-2 RNA Detection via CRISPR/Cas13a-Mediated SERS.ACS SENSORS,10(8),6253-6262. |
| MLA | Joung, Younju,et al."Dual-Pathway Lateral Flow Assay for Rapid and Sensitive SARS-CoV-2 RNA Detection via CRISPR/Cas13a-Mediated SERS".ACS SENSORS 10.8(2025):6253-6262. |
入库方式： OAI收割
来源：烟台海岸带研究所
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