Paper and cloth-based microfluidic chips for rapid cysteine detection in deep-sea cold seeps
文献类型：期刊论文
| 作者 | Hu, Die4; Xiang, Jiawen1; Guo, Jingying2; Wang, Chao1; Qi, Ji1; Li, Bowei1; Wang, Xiaoyan2; Zhang, Xin3; Chen, Lingxin1; Zhuang, Xuming4 |
| 刊名 | ANALYST
 |
| 出版日期 | 2025-05-12 |
| 卷号 | 150期号:10页码:2066-2073 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/d5an00109a |
| 通讯作者 | Li, Bowei(bwli@yic.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn) ; Zhuang, Xuming(xmzhuang@iccas.ac.cn) |
| 英文摘要 | In this study, we developed a simple and cost-effective paper and cloth-based microfluidic fluorescence sensing device for the selective and quantitative detection of l-cysteine (l-Cys) in deep-sea water, addressing the need for efficient monitoring of this critical metabolite of deep-sea creatures which acts as a biomarker for tracking these organisms. The device employs gold nanoparticles (Au NPs) immobilized on a cloth-based substrate and rhodamine B (Rh B) molecules. In the presence of l-Cys, strong interactions between l-Cys and the Au NPs release Rh B molecules, restoring fluorescence proportional to the cysteine concentration. The device achieves a low detection limit of 0.52 nM with a dynamic range of 1-100 nM. It demonstrates excellent selectivity over other amino acids, stability over 30 days, and reproducibility. Its practical applicability was confirmed using deep-sea cold seep water samples, yielding recoveries of 98.07%-102.62%. Compared to existing methods, this platform offers enhanced portability, lower cost, and faster response, making it suitable for in situ environmental monitoring. |
| WOS关键词 | COLORIMETRIC DETECTION ; GOLD NANOPARTICLES ; ASSAY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001461935700001 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China Major Scientific Research Instrument Development Project ; National Natural Science Foundation of China ; Shandong Natural Science Foundation General Project |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40861]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Li, Bowei; Zhang, Xin; Zhuang, Xuming |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Coastal Zone Ecol Environm Monitoring Technol & Eq, Yantai 264003, Shandong, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Shandong, Peoples R China 3.Laoshan Lab, Qingdao 266237, Shandong, Peoples R China 4.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Die,Xiang, Jiawen,Guo, Jingying,et al. Paper and cloth-based microfluidic chips for rapid cysteine detection in deep-sea cold seeps[J]. ANALYST,2025,150(10):2066-2073. |
| APA | Hu, Die.,Xiang, Jiawen.,Guo, Jingying.,Wang, Chao.,Qi, Ji.,...&Zhuang, Xuming.(2025).Paper and cloth-based microfluidic chips for rapid cysteine detection in deep-sea cold seeps.ANALYST,150(10),2066-2073. |
| MLA | Hu, Die,et al."Paper and cloth-based microfluidic chips for rapid cysteine detection in deep-sea cold seeps".ANALYST 150.10(2025):2066-2073. |
入库方式： OAI收割
来源：烟台海岸带研究所
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