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Intermolecular Structure Conversion-Based G4-TDF Nanostructures Functionalized μPADs for Fluorescent Determination of Potassium Ion in Serum

文献类型：期刊论文

作者Wang, Mengqi4; Fu, Xiuli4; Liu, Yixuan4; Zhang, Zhiyang3; Jiang, Chenyu2; Song, Dean1
刊名BIOSENSORS-BASEL
出版日期2025-03-31
卷号15期号:4页码:12
关键词G-quadruplex DNA tetrahedral nanostructure structure conversion thioflavin T potassium ion
DOI10.3390/bios15040223
通讯作者Jiang, Chenyu(jiangcy@sibet.ac.cn) ; Song, Dean(songdean@caas.cn)
英文摘要Herein, we proposed a versatile G-quadruplex (G4)-tetrahedral DNA framework (G4-TDF) nanostructure functionalized origami microfluidic paper-based device (mu PADs) for fluorescence detection of K+ by lighting up thioflavin T (ThT). In this work, TDF provided robust structural support for G-rich sequence in well-defined orientation and spacing to ensure high recognition efficiency, enabling sensitive fluorescence sensing on origami mu PAD. After introducing ThT, the G-rich sequences extended from TDF vertices formed a parallel G4 structure, showing weak fluorescence signal output. Upon the presence of target K+, this parallel G4 structure transitioned to antiparallel G4 structure, leading to a significantly increase in fluorescence signal of ThT. Benefiting from the outstanding fluorescence enhancement characteristic of the G4 structure for ThT and excellent specificity of the G4 structure to K+ plus satisfactory recognition efficiency with the aid of TDF, this origami paper-based fluorescence sensing strategy exhibited an impressive detection limit as low as 0.2 mM with a wide range of 0.5-5.5 mM. This innovative G4-TDF fluorescence sensing was applied for the first time on mu PAD, providing a simple, effective, and rapid method for K+ detection in human serum with significant potential for clinical diagnostics.
WOS关键词TETRAHEDRAL DNA NANOSTRUCTURE ; G-QUADRUPLEX ; SENSOR ; AMPLIFICATION ; BIOSENSOR ; NA ; CA ; MG
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001477257500001
资助机构Shandong Engineering Technology Research Laboratory of Suzhou Biomedical Engineering and Technology of Chinese Academy of Sciences ; Key R&D Program of Shandong Province, China ; National Natural Science Foundation of China
源URL[http://ir.yic.ac.cn/handle/133337/40919]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Jiang, Chenyu; Song, Dean
作者单位1.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, Coastal Zone Ecol Environm Monitoring Technol & Eq, Yantai 264003, Peoples R China
4.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Mengqi,Fu, Xiuli,Liu, Yixuan,et al. Intermolecular Structure Conversion-Based G4-TDF Nanostructures Functionalized μPADs for Fluorescent Determination of Potassium Ion in Serum[J]. BIOSENSORS-BASEL,2025,15(4):12.
APA Wang, Mengqi,Fu, Xiuli,Liu, Yixuan,Zhang, Zhiyang,Jiang, Chenyu,&Song, Dean.(2025).Intermolecular Structure Conversion-Based G4-TDF Nanostructures Functionalized μPADs for Fluorescent Determination of Potassium Ion in Serum.BIOSENSORS-BASEL,15(4),12.
MLA Wang, Mengqi,et al."Intermolecular Structure Conversion-Based G4-TDF Nanostructures Functionalized μPADs for Fluorescent Determination of Potassium Ion in Serum".BIOSENSORS-BASEL 15.4(2025):12.

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来源：烟台海岸带研究所

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