Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay
文献类型：期刊论文
| 作者 | Xu, Yuanxia1; Zhu, Yanqiao1; Cao, Jinyu1; Wang, Yanting1; Hu, Xueping1; Zhao, Xiaohua2; Song, Xingliang1; Chen, Lingxin3 |
| 刊名 | ANALYST
 |
| 出版日期 | 2025-05-27 |
| 卷号 | 150期号:11页码:2410-2419 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/d5an00291e |
| 通讯作者 | Hu, Xueping(xuephu@yeah.net) ; Zhao, Xiaohua() ; Song, Xingliang() |
| 英文摘要 | Transferrin (TRF) is an important glycoprotein for the early detection of disease. However, its rapid isolation and detection are challenging due to its complex structure, composition, and susceptibility to denaturation. Herein, a novel imprinting-based sensor was introduced to purify and detect TRF in human serum simultaneously. A magnetic molecularly imprinted sensor was developed by integrating boronate affinity-based MIPs with a boric acid-functionalized rod-like fluorescent porphyrin (TCPP) aggregate (A-TCPP-BA) to form a sandwich structure. When an alkaline solution (pH 10) was added, the TCPP was re-released due to disruption in the structure of the A-TCPP-BA, leading to a second "explosive" amplification of fluorescence signals. Therefore, sensitive detection of TRF was realized with a good linear range from 2-50 mu g mL-1 and a detection limit of 0.74 mu g mL-1. Furthermore, five pre-diagnosed serum samples were analyzed using this approach, obtaining recoveries in the range of 97.4% to 104.4%. In addition, the results of the significance tests (p-values of all five samples are greater than 0.05) were satisfactory compared with those of electrochemiluminescence (ECL). Therefore, this method shows great potential for the point-of-care testing of TRF and other glycoproteins in the future. |
| WOS关键词 | MASS-SPECTROMETRY ; RHODAMINE-B ; NANOPARTICLES ; GLYCOPROTEIN ; GLYCOSYLATION ; SEPARATION ; REMOVAL |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001480094100001 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Key Research and Development Program of Linyi City |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40922]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Hu, Xueping; Zhao, Xiaohua; Song, Xingliang |
| 作者单位 | 1.Linyi Univ, Sch Chem & Chem Engn, Linyi 276000, Peoples R China 2.Linyi Peoples Hosp, Linyi 276000, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yuanxia,Zhu, Yanqiao,Cao, Jinyu,et al. Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay[J]. ANALYST,2025,150(11):2410-2419. |
| APA | Xu, Yuanxia.,Zhu, Yanqiao.,Cao, Jinyu.,Wang, Yanting.,Hu, Xueping.,...&Chen, Lingxin.(2025).Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay.ANALYST,150(11),2410-2419. |
| MLA | Xu, Yuanxia,et al."Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay".ANALYST 150.11(2025):2410-2419. |
入库方式： OAI收割
来源：烟台海岸带研究所
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