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A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice

文献类型：期刊论文

作者Zhou, Yang5,6,7,8; Wei, Yu6,7; Yin, Jianli6,7,8,9; Kong, Deqiang6,7; Li, Wenjun1; Wang, Xinyi5,6,7; Yao, Yining4; Huang, Qin6,7; Li, Lei6,7; Liu, Mengyao6,7
刊名NUCLEIC ACIDS RESEARCH
出版日期2025-08-28
卷号53期号:15页码:18
ISSN号0305-1048
DOI10.1093/nar/gkaf758
通讯作者Ye, Haifeng(hfye@bio.ecnu.edu.cn)
英文摘要The Cre-loxP recombination system enables precise genome engineering; however, existing photoactivatable Cre tools suffer from several limitations, including low DNA recombination efficiency, background activation, slow activation kinetics, and poor tissue penetration. Here, we present REDMAPCre, a red-light-controlled split-Cre system based on the Delta PhyA/FHY1 interaction. REDMAPCre enables rapid activation (1-s illumination) and achieves an 85-fold increase in reporter expression over background levels. We demonstrate its efficient regulation of DNA recombination in mammalian cells and mice, as well as its compatibility with other inducible recombinase systems for Boolean logic-gated DNA recombination. Using a single-vector adeno-associated virus delivery system, we successfully induced REDMAPCre-mediated DNA recombination in mice. Furthermore, we generated a REDMAPCre transgenic mouse line and validated its efficient, light-dependent recombination across multiple organs. To explore its functional applications, REDMAPCre transgenic mice were crossed with isogenic Cre-dependent reporter mice, enabling optogenetic induction of insulin resistance and hepatic lipid accumulation via Cre-dependent overexpression of ubiquitin-like with PHD and RING finger domains 1 (UHRF1), as well as targeted cell ablation through diphtheria toxin fragment A expression. Collectively, REDMAPCre provides a powerful tool for achieving remote control of recombination and facilitating functional genetic studies in living systems.
WOS关键词SPATIOTEMPORAL CONTROL ; DNA ; INHIBITION ; EXPRESSION ; TAMOXIFEN ; BRAIN
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:001548837100001
资助机构National Natural Science Foundation of China ; ECNU Multifunctional Platform for Innovation
源URL[http://ir.yic.ac.cn/handle/133337/41034]  
专题海岸带生物学与生物资源利用重点实验室
通讯作者Ye, Haifeng
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Chinese Univ Hong Kong, Dept Biomed Engn, Sha Tin, Hong Kong 999077, Peoples R China
3.Beijing Life Sci Acad, Yingcai South 1st St, Beijing 102209, Peoples R China
4.East China Normal Univ, Sch Chem & Mol Engn, Dept Chem, Shanghai 200241, Peoples R China
5.East China Normal Univ, Wuhu Hosp, Hlth Sci Ctr, Wuhu 241001, Peoples R China
6.East China Normal Univ, Shanghai Frontiers Sci Ctr Genome Editing & Cell T, Inst Biomed Sci,Shanghai Key Lab Regulatory Biol, Shanghai Acad Nat Sci SANS,Biomed Synthet Biol Res, Shanghai 200241, Peoples R China
7.East China Normal Univ, Shanghai Acad Nat Sci SANS, Sch Life Sci, Shanghai 200241, Peoples R China
8.East China Normal Univ, Chongqing Inst, Chongqing Key Lab Precis Opt, Chongqing 401120, Peoples R China
9.East China Normal Univ & Fengxian Dist Cent Hosp, Joint Ctr Translat Med, Sch Life Sci, Shanghai 201499, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Yang,Wei, Yu,Yin, Jianli,et al. A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice[J]. NUCLEIC ACIDS RESEARCH,2025,53(15):18.
APA Zhou, Yang.,Wei, Yu.,Yin, Jianli.,Kong, Deqiang.,Li, Wenjun.,...&Ye, Haifeng.(2025).A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice.NUCLEIC ACIDS RESEARCH,53(15),18.
MLA Zhou, Yang,et al."A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice".NUCLEIC ACIDS RESEARCH 53.15(2025):18.

入库方式： OAI收割

来源：烟台海岸带研究所

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