SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis
文献类型：期刊论文
| 作者 | Kim, Kihyun3; Park, Sohyun3; Kang, Suyoung3; Lee, Mi-Kyung2; Chen, Lingxin1; Choo, Jaebum3 |
| 刊名 | NANO CONVERGENCE
 |
| 出版日期 | 2025-08-21 |
| 卷号 | 12期号:1页码:14 |
| 关键词 | Surface-enhanced Raman scattering
Aptasensor
Urinary tract infection
|
| ISSN号 | 2196-5404 |
| DOI | 10.1186/s40580-025-00506-0 |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr) |
| 英文摘要 | A surface-enhanced Raman scattering (SERS)-based aptasensor was developed for the rapid and sensitive detection of Escherichia coli (E. coli), a major pathogen in urinary tract infections (UTIs). The sensor utilizes magnetic beads embedded with gold nanoparticles (MB-AuNPs) functionalized with capture DNA (cDNA) as both the SERS-active substrate and magnetic separation tool. The detection mechanism relies on an aptamer DNA-probe DNA complex: when the aptamer binds specifically to E. coli, the probe DNA is released and subsequently hybridizes with cDNA on the MB-AuNPs. This brings a Cy5 Raman label close to the gold surface, generating a strong SERS signal. The assay offers a one-step process, eliminating the need for bacterial culture or nucleic acid amplification, and completes within approximately 6 h. Quantitative analysis demonstrated a detection limit of 5.9 x 103 CFU/mL, well below the clinical threshold for UTIs, with a reliable calibration curve (R2 = 0.990). Selectivity tests confirmed high specificity for E. coli without cross-reactivity to other bacteria. Clinical evaluation using 21 urine samples showed high diagnostic performance: 100% sensitivity, 91% specificity, 95% accuracy, and 100% precision compared to standard urine culture. These results highlight the aptasensor's potential as a rapid, sensitive, and specific alternative for UTI diagnosis in clinical settings. |
| WOS关键词 | GOLD NANOPARTICLES |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:001556208600001 |
| 资助机构 | National Research Foundation of Korea |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41043]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, Lingxin; Choo, Jaebum |
| 作者单位 | 1.Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Chung Ang Univ, Coll Med, Dept Lab Med, Seoul 06973, South Korea 3.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea |
| 推荐引用方式 GB/T 7714 | Kim, Kihyun,Park, Sohyun,Kang, Suyoung,et al. SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis[J]. NANO CONVERGENCE,2025,12(1):14. |
| APA | Kim, Kihyun,Park, Sohyun,Kang, Suyoung,Lee, Mi-Kyung,Chen, Lingxin,&Choo, Jaebum.(2025).SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis.NANO CONVERGENCE,12(1),14. |
| MLA | Kim, Kihyun,et al."SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis".NANO CONVERGENCE 12.1(2025):14. |
入库方式： OAI收割
来源：烟台海岸带研究所
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