Breaking Biofilm Barriers: Using CATH-ICG-Loaded Bilayer Dissolving Microneedle-Assisted Photodynamic Therapy for Deep Skin Candidiasis
文献类型：期刊论文
| 作者 | Hussain, Yaseen2; Dormocara, Amos2; Li, Huifang2; Li, Chengguo5; Khan, Muhammad Kamran3,4; Ma, Yonghao5; Leng, Gang5; Wang, Yipeng1; You, Ben-Gang2; Cui, Jing-Hao2 |
| 刊名 | MOLECULAR PHARMACEUTICS
 |
| 出版日期 | 2025-05-27 |
| 卷号 | 22期号:7页码:4101-4124 |
| 关键词 | deep skin candidiasis HcCATH-KL30 dissolvingmicroneedles biofilm photodynamic therapy |
| ISSN号 | 1543-8384 |
| DOI | 10.1021/acs.molpharmaceut.5c00367 |
| 通讯作者 | Li, Chengguo(li@youwebiotech.com) ; You, Ben-Gang(youbengang@suda.edu.cn) ; Cui, Jing-Hao(jhcui@suda.edu.cn) |
| 英文摘要 | Deep skin fungal infections, particularly biofilm-associated Candida albicans (C. albicans), pose significant clinical challenges due to their resistance to conventional antifungal therapies. The stratum corneum acts as a barrier to high molecular weight drugs, limiting the penetration of systemic and topical antifungal agents. In this study, we explored a transdermal delivery system utilizing novel Cathelicidin-HcCATH-KL30 (CATH)-loaded dissolving microneedles (DMNs) for the treatment of deep dermal C. albicans biofilm infections. Preliminary evaluations demonstrated that CATH exhibited potent antifungal activity against nonfilamentous and filamentous C. albicans but was ineffective against biofilm-embedded Candida, suggesting biofilm penetration limitations. To enhance its efficacy, we integrated indocyanine green into DMNs and applied photodynamic therapy (PDT) using near-infrared (NIR) irradiation. The generated reactive oxygen species disrupted the biofilm matrix, allowing a deeper penetration of CATH for enhanced antifungal activity. Results from in vitro, ex vivo, and in vivo models demonstrated a significant reduction (similar to 94%) in fungal burden with CATH-ICG-DMNs following NIR irradiation, highlighting a synergistic effect. Findings of the study were mechanistically validated through qRT-PCR and propodeum iodide staining, which were in accordance with the proposed hypothesis. The current research work for the first time explored the novel antimicrobial peptide from a drug delivery platform in order to investigate its potential. This study establishes a promising microneedle-based PDT strategy for combating deep skin fungal infections, overcoming biofilm-mediated resistance and enhancing antifungal therapy efficacy. |
| WOS关键词 | NANOPARTICLES ; SYSTEMS ; CANCER |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001497551000001 |
| 资助机构 | National Natural Science Foundation of China (NSFC) ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Jiangsu, China ; Suzhou International Joint Laboratory for Diagnosis and Treatment of Brain Diseases |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41094]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Li, Chengguo; You, Ben-Gang; Cui, Jing-Hao |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Peoples R China 3.Suzhou Ind Pk Monash Res Inst Sci & Technol, Suzhou 215123, Peoples R China 4.SEU Monash Joint Grad Sch, Dept Biotechnol, Suzhou 215123, Peoples R China 5.Youwe Zhuhai Biotechnol Co Ltd, Zhuhai 519085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hussain, Yaseen,Dormocara, Amos,Li, Huifang,et al. Breaking Biofilm Barriers: Using CATH-ICG-Loaded Bilayer Dissolving Microneedle-Assisted Photodynamic Therapy for Deep Skin Candidiasis[J]. MOLECULAR PHARMACEUTICS,2025,22(7):4101-4124. |
| APA | Hussain, Yaseen.,Dormocara, Amos.,Li, Huifang.,Li, Chengguo.,Khan, Muhammad Kamran.,...&Cui, Jing-Hao.(2025).Breaking Biofilm Barriers: Using CATH-ICG-Loaded Bilayer Dissolving Microneedle-Assisted Photodynamic Therapy for Deep Skin Candidiasis.MOLECULAR PHARMACEUTICS,22(7),4101-4124. |
| MLA | Hussain, Yaseen,et al."Breaking Biofilm Barriers: Using CATH-ICG-Loaded Bilayer Dissolving Microneedle-Assisted Photodynamic Therapy for Deep Skin Candidiasis".MOLECULAR PHARMACEUTICS 22.7(2025):4101-4124. |
入库方式： OAI收割
来源：烟台海岸带研究所
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