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A C1q domain-containing protein from Scapharca subcrenata may participate in the immune defense against pathogen infection

文献类型：期刊论文

作者Yang, Jing2,3; Peng, Qiang4; Shen, Min5; Liu, Guilong1; Xu, Xin1; Wang, Chunde1,2; Ning, Junhao2
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2025-09-01
卷号164页码:9
关键词C1qDCs Scapharca subcrenata Immune defense RNA interference Expression profiles
ISSN号1050-4648
DOI10.1016/j.fsi.2025.110438
通讯作者Wang, Chunde(chundewang2007@163.com) ; Ning, Junhao(jhning@yic.ac.cn)
英文摘要The C1q domain-containing proteins (C1qDCs) have been shown to play crucial roles in immune responses among invertebrates, but few studies have focused on immune regulatory pathways in bivalves. In this study, we identified a C1qDC gene with a typical C1q domain (designated as SsC1qDC1) from Scapharca subcrenata and characterized its potential immune response against pathogen infection. The full-length cDNA sequence of SsC1qDC1 was 870 bp, encoding a peptide of 259 amino acids with an N-terminal signal peptide. Sequence analysis indicated that SsC1qDC1 was not conserved compared with C1qDCs from other bivalves. SsC1qDC1 was detected in all examined tissues, with higher expression levels in hepatopancreas, mantle, and gill. Concurrently, the expressions of SsC1qDC1 increased significantly from eggs to the gastrula stage and increased remarkably following Vibrio parahaemolyticus challenge. RNA interference-mediated knockdown of SsC1qDC1 significantly repressed the expressions of maternally derived immune-related genes (Vg, Lysozyme, Dscam, TEP, complement C3, and C1qDC3), while remarkably augmenting the expression of phenoloxidase activation factor PAF3. Furthermore, compared to the controls, the phagocytosis rate and total number of hemocytes were markedly reduced in the SsC1qDC1-silenced ark shells post V. parahaemolyticus infection, and thus, a significant increase in mortality rate and hemocyte apoptotic levels. Collectively, these findings implied that SsC1qDC1 may play crucial roles in immune defense against pathogen infection in ark shells.
WOS关键词PATTERN-RECOGNITION RECEPTOR ; FUNCTIONAL-ANALYSIS ; CRASSOSTREA-GIGAS ; COMPLEMENT-SYSTEM ; INNATE IMMUNITY ; PHAGOCYTOSIS ; EXPRESSION ; APOPTOSIS ; REVEALS ; PATHWAY
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:001500145000002
资助机构National Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System
源URL[http://ir.yic.ac.cn/handle/133337/41103]  
专题海岸带生物学与生物资源利用重点实验室
通讯作者Wang, Chunde; Ning, Junhao
作者单位1.Yantai Spring Sea AquaSeed Co Ltd, Yantai 264006, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
5.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Jing,Peng, Qiang,Shen, Min,et al. A C1q domain-containing protein from Scapharca subcrenata may participate in the immune defense against pathogen infection[J]. FISH & SHELLFISH IMMUNOLOGY,2025,164:9.
APA Yang, Jing.,Peng, Qiang.,Shen, Min.,Liu, Guilong.,Xu, Xin.,...&Ning, Junhao.(2025).A C1q domain-containing protein from Scapharca subcrenata may participate in the immune defense against pathogen infection.FISH & SHELLFISH IMMUNOLOGY,164,9.
MLA Yang, Jing,et al."A C1q domain-containing protein from Scapharca subcrenata may participate in the immune defense against pathogen infection".FISH & SHELLFISH IMMUNOLOGY 164(2025):9.

入库方式： OAI收割

来源：烟台海岸带研究所

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