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  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Surface enhanced Raman scattering tag enabled ultrasensitive molecular identification of Hippocampus trimaculatus based on DNA barcoding

文献类型：期刊论文

作者Wang, Qishuo2; Wu, Yixuan1; Wang, Yunqing1; Mei, Rongchao4; Zhao, Rui2; Wang, Xiaoyan4; Chen, Lingxin1,3
刊名TALANTA
出版日期2025-11-01
卷号294页码:8
关键词Surface enhanced Raman scattering DNA Polymerase chain reaction PCR-SERS assay Point-of-care
ISSN号0039-9140
DOI10.1016/j.talanta.2025.128289
通讯作者Wang, Yunqing(yqwang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Rapid and precise DNA barcode-based identification of biological species holds significant potential for pharmaceutical authentication and biomedical diagnostics. Herein, we present a polymerase chain reaction (PCR)- surface-enhanced Raman scattering (SERS) platform that integrates SERS tags for ultrasensitive and fast authentication of Hippocampus trimaculatus, a high-value traditional Chinese medicine (TCM). The SERS tags are composed of gold nanostars, near-infrared cyanine7 Raman reporters and carboxylated polystyrene shells, which achieve single-particle detection sensitivity under 780 nm irradiation. The tags also show excellent colloidal and SERS stability under physiologically relevant conditions (e.g., phosphate buffer saline, serum, 1 mM NaCl, and pH 1-12), with signal variations less than 5 %. The carboxylated polystyrene shells enable efficient DNA functionalization. Leveraging these advancements, the PCR-SERS assay detects genomic DNA (gDNA) at concentrations as low as 10 copies/mu L within 20 thermal cycles, with remarkable specificity for Hippocampus trimaculatus over four common adulterant species. Notably, the method reduces amplification requirements to 5 thermal cycles (detection limit of 106 copies/mu L) while completing the entire workflow in less than 30 min (conventional qPCR, 20-30 cycles, 1-2 h). Beyond TCM verification, this PCR-SERS platform holds broad applicability for rapid nucleic acid detection in fields ranging from environmental eDNA monitoring to point-of-care diagnostics.
WOS关键词MULTIPLEX DETECTION ; MUTATIONS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001509226500001
资助机构Key R & D Program of Shandong Province, China ; National Natural Sci-ence Foundation of China ; Taishan Scholar Project Special Funding ; Special Fund for Leading Scholar Program of Yantai
源URL[http://ir.yic.ac.cn/handle/133337/41121]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Wang, Yunqing; Chen, Lingxin
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Shandong Univ Tradit Chinese Med, Res Inst Marine Tradit Chinese Med, Qingdao 266112, Peoples R China
3.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Qishuo,Wu, Yixuan,Wang, Yunqing,et al. Surface enhanced Raman scattering tag enabled ultrasensitive molecular identification of Hippocampus trimaculatus based on DNA barcoding[J]. TALANTA,2025,294:8.
APA Wang, Qishuo.,Wu, Yixuan.,Wang, Yunqing.,Mei, Rongchao.,Zhao, Rui.,...&Chen, Lingxin.(2025).Surface enhanced Raman scattering tag enabled ultrasensitive molecular identification of Hippocampus trimaculatus based on DNA barcoding.TALANTA,294,8.
MLA Wang, Qishuo,et al."Surface enhanced Raman scattering tag enabled ultrasensitive molecular identification of Hippocampus trimaculatus based on DNA barcoding".TALANTA 294(2025):8.

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来源：烟台海岸带研究所

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