Novel antimicrobial peptide ECCT from Chinese water snake is a potential candidate for the treatment of Helicobacter pylori infection and gastric cancer
文献类型：期刊论文
| 作者 | Qiu, Jie3; Shi, Wenzhuang1,2; Qian, Zhenghai3; Zhang, Pengyu3; Li, Shuangyu1,2; Shen, Genhai3; Wang, Yipeng1,2; Li, Bin3 |
| 刊名 | MICROBIAL PATHOGENESIS
 |
| 出版日期 | 2025-09-01 |
| 卷号 | 206页码:13 |
| 关键词 | Enhydris chinensis Cathelicidin Anti-H. pylori Anti-Inflammation Anti-Gastric cancer |
| ISSN号 | 0882-4010 |
| DOI | 10.1016/j.micpath.2025.107821 |
| 通讯作者 | Shen, Genhai(ghshen@suda.edu.cn) ; Wang, Yipeng(ypwang@yic.ac.cn) ; Li, Bin(bli4004@suda.edu.cn) |
| 英文摘要 | Background: Helicobacter pylori (H. pylori) is a highly virulent bacterial pathogen that exhibits close association with the development of duodenal and gastric ulcers, as well as carcinomas. Several antimicrobial peptides (AMPs) have demonstrated remarkable efficacy in combating H. pylori infections. However, limited studies have been conducted on AMPs possessing anti-H. pylori activity. Herein, we report the identification and characterization of ECCT, a novel cathelicidins family AMP derived from the Chinese water snake Enhydris chinensis. Materials and methods: We identified a novel antimicrobial peptide, ECCT, through cDNA sequencing. The phylogeny and secondary structure of ECCT were analyzed using the neighborhood connection method. The MIC of ECCT against various pathogens was determined, and the inhibitory effect on proinflammatory cytokine transcription was detected using qPCR. Furthermore, the effects of ECCT on the MAPK inflammatory pathway induced by Helicobacter pylori were analyzed via Western Blotting. Lastly, the cytotoxic effects of ECCT on various human gastric cancer cell lines were evaluated using the MTT assay. Results: ECCT exhibits potent and rapid bactericidal activity against H. pylori, with minimum inhibitory concentrations (MICs) ranging from 1.3 to 5.1 mu M and complete bacterial eradication within 10 min. Additionally, ECCT demonstrates robust anti-inflammatory effects against H. pylori infection by significantly reducing the transcription and production of pro-inflammatory cytokines through inhibition of the MAPK signaling pathway. Importantly, ECCT also effectively induces apoptosis in gastric cancer cells at low concentrations and causes direct lysis at high concentrations, while maintaining low toxicity to normal cells. Conclusion: These findings underscore the potential of ECCT as a promising therapeutic approach for preventing and treating gastritis and gastric cancer caused by H. pylori infections. Particularly, this study highlights the multifaceted nature of ECCT as a versatile therapeutic agent, offering a novel strategy for addressing a spectrum of H. pylori-related gastric diseases. |
| WOS关键词 | HOST-DEFENSE PEPTIDES ; PATTERN-RECOGNITION ; INNATE ; CATHELICIDINS ; IDENTIFICATION ; RESISTANCE ; MECHANISMS ; IMMUNITY |
| WOS研究方向 | Immunology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001518608200003 |
| 资助机构 | Gusu Health Top-Notch Youth Talent of Suzhou Health Commission ; Suzhou Science and Technology Bureau ; Health Key Talent of Wujiang Health Commission ; Key Research & Development Plan in Social Development of Jiangsu Province ; Suzhou Agricultural Science and Technology Innovation Project |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41160]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Shen, Genhai; Wang, Yipeng; Li, Bin |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Jiangsu, Peoples R China 3.Soochow Univ, Dept Gen Surg, Suzhou Hosp 9, Suzhou 215000, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Jie,Shi, Wenzhuang,Qian, Zhenghai,et al. Novel antimicrobial peptide ECCT from Chinese water snake is a potential candidate for the treatment of Helicobacter pylori infection and gastric cancer[J]. MICROBIAL PATHOGENESIS,2025,206:13. |
| APA | Qiu, Jie.,Shi, Wenzhuang.,Qian, Zhenghai.,Zhang, Pengyu.,Li, Shuangyu.,...&Li, Bin.(2025).Novel antimicrobial peptide ECCT from Chinese water snake is a potential candidate for the treatment of Helicobacter pylori infection and gastric cancer.MICROBIAL PATHOGENESIS,206,13. |
| MLA | Qiu, Jie,et al."Novel antimicrobial peptide ECCT from Chinese water snake is a potential candidate for the treatment of Helicobacter pylori infection and gastric cancer".MICROBIAL PATHOGENESIS 206(2025):13. |
入库方式： OAI收割
来源：烟台海岸带研究所
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