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Selection of azaspiracid-1 aptamers for potentiometric aptasensing

文献类型：期刊论文

作者Zhao, Haijie1,2; Liu, Zhe1,2; Li, Jinghui2; Yin, Tanji2; Qin, Wei2,3,4
刊名TALANTA
出版日期2026
卷号296页码:9
关键词Azaspiracid-1 Aptamer MRGO-SELEX Potentiometric aptasensing
ISSN号0039-9140
DOI10.1016/j.talanta.2025.128407
通讯作者Yin, Tanji(tjyin@yic.ac.cn) ; Qin, Wei(wqin@yic.ac.cn)
英文摘要Azaspiracids (AZAs) are polyether marine toxins from shellfish that cause risks to human health. The bioreceptors for biosensing of AZAs are limited to antibodies. The selection of aptamers that specifically bind to AZAs with high affinities is highly required. In this work, aptamers with high affinities and specificities towards AZA1, which is the most abundant toxin in the AZAs group, have been selected via the magnetic reduced graphene oxide-based systematic evolution of ligands by exponential enrichment (MRGO-SELEX). A truncated aptamer (Apt-23b48) with a dissociation constant (Kd) of 29.8 +/- 8.3 nM has been chosen as a bioreceptor for fabricating DNA self-assembled nanostructure-based magnetic beads via a hybridization chain reaction. A polycation-sensitive thin-membrane electrode using protamine as an indicator has been employed to detect the surface charge change of the DNA self-assembled nanostructure-based magnetic beads induced by the binding interaction between the selected aptamer and AZA1. The proposed potentiometric aptasensing platform shows a linear range of 10-250 pM with a detection limit of 8.5 pM, and has been successfully applied to the detection of AZA1 in spike seawater samples.
WOS关键词MARINE BIOTOXINS ; HIGH-AFFINITY ; SPECIFICITY ; ENRICHMENT ; SHELLFISH
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001511695900003
资助机构Key R&D Program of Shandong Province, China ; National Natural Science Foundation of China ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province ; National Science Foundation of Shandong Province
源URL[http://ir.yic.ac.cn/handle/133337/41182]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Yin, Tanji; Qin, Wei
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Haijie,Liu, Zhe,Li, Jinghui,et al. Selection of azaspiracid-1 aptamers for potentiometric aptasensing[J]. TALANTA,2026,296:9.
APA Zhao, Haijie,Liu, Zhe,Li, Jinghui,Yin, Tanji,&Qin, Wei.(2026).Selection of azaspiracid-1 aptamers for potentiometric aptasensing.TALANTA,296,9.
MLA Zhao, Haijie,et al."Selection of azaspiracid-1 aptamers for potentiometric aptasensing".TALANTA 296(2026):9.

入库方式： OAI收割

来源：烟台海岸带研究所

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