Optimum extraction and energy transfer dynamics in Phycocyanin 620 from Thermosynechococcus vulcanus
文献类型：期刊论文
| 作者 | Pu, Yang3; Lan, Xiangyu3; Wang, Jiayu2,4; Liu, Chuanxu3; Ding, Juan1; Li, Wenjun5 |
| 刊名 | DYES AND PIGMENTS
 |
| 出版日期 | 2025-10-01 |
| 卷号 | 241页码:9 |
| 关键词 | Phycocyanin 620 Analytical grade purity Structural and functional integrity Energy transfer dynamics Transient absorption spectra |
| ISSN号 | 0143-7208 |
| DOI | 10.1016/j.dyepig.2025.112919 |
| 通讯作者 | Ding, Juan(ldu-dj@ldu.edu.cn) ; Li, Wenjun(wjli@yic.ac.cn) |
| 英文摘要 | Phycocyanin 620 (PC620) is a water-soluble blue phycobiliprotein (PBP) originating from hot spring cyanobacteria - Thermosynechococcus vulcanus (Tv). It has been recognized as a valuable material with great potential for application to theoretical studies and commercial products. So, in our work, the PC620 was purified by a minimalistic procedure through hydrophobic chromatography and used for the study of excitation energy transfer (EET) characterization. The results show that the obtained PC620 with a final recovery of 81.5 % and an analytical grade purity at 5.21 (A(620)/A(280)) was reached through the process that consisted of liquid nitrogen grinding, 20 %-50 % ammonium sulfate fractionation and one-step hydrophobic interaction chromatography. The final PC620 showed a maximum absorption peak at 620 nm, a fluorescence emission peak at 652 nm and a positive peak of circular dichroism (CD) spectra in the visible range at 626 nm. Then, joint analysis of absorption spectra, fluorescence spectra, and CD spectra confirmed that the structural and functional integrity of the samples was retained compared with natural PC620. In addition, the exciton coupling in PC620 has been excluded by CD measurements. Furthermore, in an accurate experimental determination of the EET lifetime in PC620, two energy migration pathways from alpha 84 to beta 84 in a period of similar to 0.25 ps and then from beta 155 to beta 84 in a period of similar to 5 ps were directly resolved by ultrafast time-resolved spectroscopy. The calculated results indicated that the EET process in PC620 is dominated by the F & ouml;rster resonance energy transfer (FRET) mechanism. This work should be conducive to the highly efficient preparation of the analytical grade PC620 using a substantially simpler purification method for exploring the applications of theoretical research and the commercial field. |
| WOS关键词 | CYANOBACTERIUM SYNECHOCOCCUS-VULCANUS ; C-PHYCOCYANIN ; CRYSTAL-STRUCTURE ; THERMAL-STABILITY ; PHOTOSYSTEM-II ; RED ALGAE ; PHYCOBILIPROTEINS ; ALLOPHYCOCYANIN ; PHYCOBILISOMES ; PHYCOERYTHRIN |
| WOS研究方向 | Chemistry ; Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001516262200002 |
| 资助机构 | National Key Research and Devel-opment Program of China ; Natural Science Foundation of Shandong Province |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41185]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Ding, Juan; Li, Wenjun |
| 作者单位 | 1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ludong Univ, Sch Fisheries, Yantai 264025, Peoples R China 4.Chinese Acad Sci, Lab Soft Matter Phys, Inst Phys, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Yang,Lan, Xiangyu,Wang, Jiayu,et al. Optimum extraction and energy transfer dynamics in Phycocyanin 620 from Thermosynechococcus vulcanus[J]. DYES AND PIGMENTS,2025,241:9. |
| APA | Pu, Yang,Lan, Xiangyu,Wang, Jiayu,Liu, Chuanxu,Ding, Juan,&Li, Wenjun.(2025).Optimum extraction and energy transfer dynamics in Phycocyanin 620 from Thermosynechococcus vulcanus.DYES AND PIGMENTS,241,9. |
| MLA | Pu, Yang,et al."Optimum extraction and energy transfer dynamics in Phycocyanin 620 from Thermosynechococcus vulcanus".DYES AND PIGMENTS 241(2025):9. |
入库方式： OAI收割
来源：烟台海岸带研究所
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