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  4. 海岸带生物学与生物资源利用重点实验室
Regulation of enzymatic activity and NADPH supply for efficient biosynthesis of phycocyanin in Escherichia coli

文献类型：期刊论文

作者Chen, Xin3,4; Chi, Menghan3,4; Du, Jingjing3,4; Guo, Hongda3,4; Zhao, Yabin2; Lv, Kangning3,4; Wang, Shuo3,4; Tang, Zhihong2; Li, Wenjun1; Ge, Baosheng3,4
刊名MOLECULAR CATALYSIS
出版日期2025-10-01
卷号585页码:7
关键词Phycocyanobilin Escherichia coli Combinational biosynthesis Fed batch fermentation
ISSN号2468-8231
DOI10.1016/j.mcat.2025.115334
通讯作者Ge, Baosheng(gebaosheng@upc.edu.cn)
英文摘要Phycocyanobilin (PCB) offers not only unique blue color but also excellent antioxidant and photosensitizing properties, which can be widely used in the field of food and medicine. However, the biosynthesis of PCB using genetically engineered E. coli has been plagued by low yield due to the lower enzymatic activity and substrate/ NADPH supply. In this study, two key genes of Ho1 and PcyA from different sources were screened for efficient biosynthesis of PCB, and the glucose dehydrogenase (GDH) were also introduced to accelerate the NADPH regeneration in E. coli. The construct can enhance the yield of PCB to 9.5 mg/L. After optimization, the PCB yield was increased to 29.43 mg/L in 5 L fermenter using the feeding batch strategy. Furthermore, an in vitro PCB catalytic synthesis system was constructed, which can result in a PCB yield as 19.9 mg/L in 3.5 h. This study provides useful strategies for efficient PCB synthesis using genetical techniques.
WOS关键词C-PHYCOCYANIN ; OXIDOREDUCTASE ; REGENERATION ; CHROMOPHORES ; CLEAVAGE
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001530723700001
资助机构National Natural Science Foundation of China ; Major basic research plan of Shandong Province ; Inner Mongolia Autonomous Region Science and Technology Project ; Qingdao West Coast New Area Technology Project
源URL[http://ir.yic.ac.cn/handle/133337/41224]  
专题海岸带生物学与生物资源利用重点实验室
通讯作者Ge, Baosheng
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
2.Yantai Univ, Coll Life Sci, Yantai 264003, Peoples R China
3.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
4.China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xin,Chi, Menghan,Du, Jingjing,et al. Regulation of enzymatic activity and NADPH supply for efficient biosynthesis of phycocyanin in Escherichia coli[J]. MOLECULAR CATALYSIS,2025,585:7.
APA Chen, Xin.,Chi, Menghan.,Du, Jingjing.,Guo, Hongda.,Zhao, Yabin.,...&Ge, Baosheng.(2025).Regulation of enzymatic activity and NADPH supply for efficient biosynthesis of phycocyanin in Escherichia coli.MOLECULAR CATALYSIS,585,7.
MLA Chen, Xin,et al."Regulation of enzymatic activity and NADPH supply for efficient biosynthesis of phycocyanin in Escherichia coli".MOLECULAR CATALYSIS 585(2025):7.

入库方式： OAI收割

来源：烟台海岸带研究所

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