Fluorescence-free near-infrared nanoprobes for in vivo resonant Raman imaging
文献类型：期刊论文
| 作者 | Fu, Guowei; Li, Jin |
| 刊名 | CHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2025-10-15 |
| 卷号 | 522页码:9 |
| 关键词 | Black hole quencher SERRS nanotags Photostability Signal-to-background ratio CXCR4 imaging |
| ISSN号 | 1385-8947 |
| DOI | 10.1016/j.cej.2025.167709 |
| 通讯作者 | Li, Jin(jinli@yic.ac.cn) |
| 英文摘要 | Near-infrared (NIR) surface-enhanced resonance Raman scattering (SERRS) nanotags have gained significant attention in bioimaging. However, the resonant Raman signals of the nanoprobes is always accompanied by fluorescence backgrounds, which results in low signal-to-background ratio (SBR) and photo-instability caused by photobleaching, thus limiting Raman imaging quality in vivo. In this study, we employed a black hole quencher as reporters to fabricate bright and nonfluorescent NIR-resonant SERRS nanotags. The resultant nanoprobes demonstrated near-zero fluorescent background, femtomolar sensitivity (15 fM), high SBR (30.87) and unparalleled photostability (time constant of 18,792 s) without fluorescence photobleaching, outperforming that of fluorescent counterparts with ICG as dyes. After functionalized with a chemokine receptor 4 (CXCR4) antagonist, the SERRS nanoprobes allowed for photostable and quantitative imaging of CXCR4 expression (a hallmark of breast cancer) in vitro at the single-cell level, and in vivo with high SBR. Moreover, the nanoprobes could clearly depict tumor margins without ambiguity, favorable for precise surgical navigation. We envision that the nonfluorescent resonant Raman nanoprobes will find broad applications in imaging and sensing in vivo. |
| WOS关键词 | SINGLE-MOLECULE ; SIGNAL ; TIME ; FLUOROPHORES ; SCATTERING |
| WOS研究方向 | Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:001566382100027 |
| 资助机构 | NSFC |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41343]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Li, Jin |
| 作者单位 | Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Guowei,Li, Jin. Fluorescence-free near-infrared nanoprobes for in vivo resonant Raman imaging[J]. CHEMICAL ENGINEERING JOURNAL,2025,522:9. |
| APA | Fu, Guowei,&Li, Jin.(2025).Fluorescence-free near-infrared nanoprobes for in vivo resonant Raman imaging.CHEMICAL ENGINEERING JOURNAL,522,9. |
| MLA | Fu, Guowei,et al."Fluorescence-free near-infrared nanoprobes for in vivo resonant Raman imaging".CHEMICAL ENGINEERING JOURNAL 522(2025):9. |
入库方式： OAI收割
来源：烟台海岸带研究所
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