Paper-based fluorescence chip with umbellately anchored dimeric G-quadruplex coupled DNA tetrahedron-structured probe for sensing DNA methyltransferase
文献类型：期刊论文
| 作者 | Wang, Mengqi4; Qi, Ji3; Li, Fengling4; Liu, Yixuan4; Xiang, Jiawen3; Li, Bowei3; Zhuang, Xuming4; Chen, Lingxin1,2,3; Fu, Xiuli4 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2026 |
| 卷号 | 446页码:8 |
| 关键词 | G-quadruplex dimer DNA tetrahedron Microfluidic paper-based device DNA methyltransferase Fluorescence analysis |
| DOI | 10.1016/j.snb.2025.138643 |
| 通讯作者 | Qi, Ji(jqi@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn) ; Fu, Xiuli(fuxiuli@ytu.edu.cn) |
| 英文摘要 | To overcome inaccessible active sites and enhance the density of sensing hotspots at limited active sites on paper-based chips, a G-quadruplex (G4) dimer-coupled DNA tetrahedron (DT) fluorescent probe was umbellately anchored on a dual-channel origami microfluidic paper device (mu PAD). Taking DNA adenine methylation MTase (Dam MTase), an early biomarker for various cancers, as a model target, Dam MTase recognized and catalyzed the methylation of its substrate, which initiated a cleavage reaction mediated by the restriction endonuclease DpnI. This process, enabled by the umbellate probe configuration, released a large quantity of G4 dimer sequences, reducing their quantity on the mu PAD and causing a corresponding decrease in fluorescence signal. The strategy leveraged the rich signal molecular sites of the DT achieved through the umbellate anchoring approach, the high specificity of the Dam MTase/endonuclease system, the excellent fluorescence enhancement properties of the G4 dimer structure with thioflavin T, and the outstanding mechanical properties of the DT. As a result, the method achieved a detection limit as low as 0.11 U/mL and a wide linear detection range from 1-90 U/mL. Furthermore, this approach was successfully applied to detect Dam MTase activity in serum. The proposed method shows great potential for disease diagnosis and clinical research. |
| WOS关键词 | METHYLATION ; STRATEGY ; ASSAY ; AMPLIFICATION ; BIOSENSOR |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:001568960700022 |
| 资助机构 | National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation Key Project ; National Natural Science Foundation of China Major Scientific Research Instrument Development Project ; Shandong Provincial Natural Science Foundation |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41380]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Qi, Ji; Chen, Lingxin; Fu, Xiuli |
| 作者单位 | 1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Shaoxing Univ, Inst Adv Study, Shaoxing 312000, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zone Ecol Environm Monitoring Technol & Eq, Shandong Key Lab Coastal Environm Proc,Key Lab, Yantai 264003, Peoples R China 4.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Mengqi,Qi, Ji,Li, Fengling,et al. Paper-based fluorescence chip with umbellately anchored dimeric G-quadruplex coupled DNA tetrahedron-structured probe for sensing DNA methyltransferase[J]. SENSORS AND ACTUATORS B-CHEMICAL,2026,446:8. |
| APA | Wang, Mengqi.,Qi, Ji.,Li, Fengling.,Liu, Yixuan.,Xiang, Jiawen.,...&Fu, Xiuli.(2026).Paper-based fluorescence chip with umbellately anchored dimeric G-quadruplex coupled DNA tetrahedron-structured probe for sensing DNA methyltransferase.SENSORS AND ACTUATORS B-CHEMICAL,446,8. |
| MLA | Wang, Mengqi,et al."Paper-based fluorescence chip with umbellately anchored dimeric G-quadruplex coupled DNA tetrahedron-structured probe for sensing DNA methyltransferase".SENSORS AND ACTUATORS B-CHEMICAL 446(2026):8. |
入库方式： OAI收割
来源：烟台海岸带研究所
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