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Comparative Transcriptomics Reveals Distinct Adaptation Mechanisms for Degradation of n-Alkane and Branched Alkane in the Salt-Tolerant Bacterium Dietzia sp. CN-3

文献类型：期刊论文

作者Chen, Weiwei2; Sun, Jiawei2; Zhang, Xin2; Zhang, Jiawen2; Wang, Yuan1; Cheng, Shiwei2
刊名MICROORGANISMS
出版日期2025-09-20
卷号13期号:9页码:21
关键词comparative transcriptomics n-alkane branched alkane Dietzia
DOI10.3390/microorganisms13092206
通讯作者Cheng, Shiwei(swcheng@ldu.edu.cn)
英文摘要Although hydrocarbon-degrading bacteria utilize a wide range of alkanes, the global metabolic features and regulatory mechanisms governing their growth on alkanes with different chain lengths remain incompletely elucidated. In this study, we analyzed the comparative transcriptomics of a salt-tolerant bacterium, Dietzia sp. CN-3, to investigate molecular adaptations and metabolic processes when grown on n-hexadecane (C16), branched alkane (pristane), and glucose. A total of 1766 differentially expressed genes (DEGs) were identified in the C16 group compared to the glucose control, with 1024 (58.0%) being upregulated and 742 (42.0%) being downregulated. Notably, the pristane group exhibited 1542 DEGs, of which 488 (31.6%) were upregulated and 1054 (68.4%) were downregulated. Our results demonstrate that C16 and pristane induced common genes of alkane hydroxylation in the core alkane degradation pathway, while eliciting distinct transcriptional patterns of genes involved in lipid metabolism, energy metabolism, metal ion transportation, cell surface composition biosynthesis, and transcription regulation. The findings reveal that CN-3 employs diverse metabolic strategies to adapt to alkanes with different chain lengths, displaying considerable metabolic plasticity. This study significantly enhances our understanding of molecular adaptation of bacteria to hydrocarbon-containing environments and may provide valuable information for further studies of petroleum hydrocarbon bioremediation.
WOS关键词DEGRADING BACTERIUM ; CRUDE-OIL ; FAMILY ; GENE ; DQ12-45-1B ; DIVERSITY ; PATHWAY ; SYSTEMS
WOS研究方向Microbiology
语种英语
WOS记录号WOS:001580623900001
资助机构National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation
源URL[http://ir.yic.ac.cn/handle/133337/41401]  
专题海岸带生物学与生物资源利用重点实验室
通讯作者Cheng, Shiwei
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Weiwei,Sun, Jiawei,Zhang, Xin,et al. Comparative Transcriptomics Reveals Distinct Adaptation Mechanisms for Degradation of n-Alkane and Branched Alkane in the Salt-Tolerant Bacterium Dietzia sp. CN-3[J]. MICROORGANISMS,2025,13(9):21.
APA Chen, Weiwei,Sun, Jiawei,Zhang, Xin,Zhang, Jiawen,Wang, Yuan,&Cheng, Shiwei.(2025).Comparative Transcriptomics Reveals Distinct Adaptation Mechanisms for Degradation of n-Alkane and Branched Alkane in the Salt-Tolerant Bacterium Dietzia sp. CN-3.MICROORGANISMS,13(9),21.
MLA Chen, Weiwei,et al."Comparative Transcriptomics Reveals Distinct Adaptation Mechanisms for Degradation of n-Alkane and Branched Alkane in the Salt-Tolerant Bacterium Dietzia sp. CN-3".MICROORGANISMS 13.9(2025):21.

入库方式： OAI收割

来源：烟台海岸带研究所

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