Identification, expression, and functional analysis of a chitin-degrading enzyme CtChi70 for N-acetyl Chitobiose production
文献类型：期刊论文
| 作者 | Zhang, Shiqing2; Lin, Yifan2; Zhang, Zhanhua2; Ou, Xingyan2; Xu, Zhenbo3; Zou, Chengjuan4; Soteyome, Thanapop5; Li, Lili1; Lin, Xiaomei3; Ye, Yanrui2 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2025-11-01 |
| 卷号 | 330页码:8 |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2025.147953 |
| 通讯作者 | Li, Lili(llli@yic.ac.cn) ; Lin, Xiaomei(linxm24@scut.edu.cn) ; Ye, Yanrui(esyanruiye@scut.edu.cn) |
| 英文摘要 | Chitin, the second most abundant natural polysaccharide, is a renewable resource with great potential for producing functional oligosaccharides. In this study, we identified and characterized CtChi70, a glycoside hydrolase family 18 (GH18) exochitinase that selectively hydrolyzes chitin to produce N-acetyl chitobiose [(GlcNAc)(2)] with exceptional purity (>= 98 %). CtChi70 was heterologously expressed in Escherichia coli CtChi70 at a high yield of 228.2 +/- 47.8 mg L-1. The purified enzyme exhibited optimal activity at pH 5.0 and 45 degrees C, and demonstrated stability across a broad pH range (4.0-12.0). Time-course hydrolysis of colloidal chitin showed moderate catalytic efficiency but high product specificity, peaking at 24 h with minimal N-acetylglucosamine byproduct. The ability of CtChi70 to directly convert raw chitin powder to high-purity (GlcNAc)(2) suggests its potential for applications requiring pure chitooligosaccharides. These findings highlight CtChi70 as a promising biocatalyst for sustainable chitin valorization. |
| WOS关键词 | PURIFICATION ; CLONING ; WASTE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001589679800001 |
| 资助机构 | Postdoctoral Fellowship Program (Grade B) of China Postdoctoral Science Foundation ; Natural Science Foundation of Guangdong Province of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41465]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Li, Lili; Lin, Xiaomei; Ye, Yanrui |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Peoples R China 3.South China Univ Technol, Engn Res Ctr Starch & Vegetable Prot Proc, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Sch Food Sci & Engn,Minist Educ, Guangzhou 510640, Peoples R China 4.Sino Singapore Int Joint Res Inst, Guangzhou 510555, Peoples R China 5.Rajamangala Univ Technol Phra Nakhon, Home Econ Technol, Bangkok, Thailand |
| 推荐引用方式 GB/T 7714 | Zhang, Shiqing,Lin, Yifan,Zhang, Zhanhua,et al. Identification, expression, and functional analysis of a chitin-degrading enzyme CtChi70 for N-acetyl Chitobiose production[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2025,330:8. |
| APA | Zhang, Shiqing.,Lin, Yifan.,Zhang, Zhanhua.,Ou, Xingyan.,Xu, Zhenbo.,...&Ye, Yanrui.(2025).Identification, expression, and functional analysis of a chitin-degrading enzyme CtChi70 for N-acetyl Chitobiose production.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,330,8. |
| MLA | Zhang, Shiqing,et al."Identification, expression, and functional analysis of a chitin-degrading enzyme CtChi70 for N-acetyl Chitobiose production".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 330(2025):8. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。