The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501
文献类型：期刊论文
| 作者 | Zhan, Yuhua1; Yan, Yongliang1; Deng, Zhiping1; Chen, Ming1; Lu, Wei1; Lu, Chao1; Shang, Liguo1; Yang, Zhimin1; Zhang, Wei1; Wang, Wei1 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2016-07-26 |
| 卷号 | 113期号:30页码:E4348-E4356 |
| 关键词 | Nitrogen Fixation Pseudomonas Stutzeri Regulatory Ncrna Nfis Nifk Mrna |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1604514113 |
| 文献子类 | Article |
| 英文摘要 | Unlike most Pseudomonas, the root-associated bacterium Pseudomonas stutzeri A1501 fixes nitrogen after the horizontal acquisition of a nitrogen-fixing (nif) island. A genome-wide search for small noncoding RNAs (ncRNAs) in P. stutzeri A1501 identified the novel P. stutzeri-specific ncRNA NfiS in the core genome, whose synthesis was significantly induced under nitrogen fixation or sorbitol stress conditions. The expression of NfiS was RNA chaperone Hfq-dependent and activated by the sigma factor RpoN/global nitrogen activator NtrC/nif-specific activator NifA regulatory cascade. The nfiS-deficient mutant displayed reduced nitrogenase activity, as well as increased sensitivity to multiple stresses, such as osmotic and oxidative stresses. Secondary structure prediction and complementation studies confirmed that a stem-loop structure was essential for NfiS to regulate the nitrogenase gene nifK mRNA synthesis and thus nitrogenase activity. Microscale thermophoresis and physiological analysis showed that NfiS directly pairs with nifK mRNA and ultimately enhances nitrogenase activity by increasing the translation efficiency and the half-life of nifK mRNA. Our data also suggest structural and functional divergence of NfiS evolution in diazotrophic and nondiazotrophic backgrounds. It is proposed that NfiS was recruited by nifK mRNA as a novel regulator to integrate the horizontally acquired nif island into host global networks. |
| WOS关键词 | ESCHERICHIA-COLI ; BIOFILM FORMATION ; SINORHIZOBIUM-MELILOTI ; AZOTOBACTER-VINELANDII ; STRESS-RESPONSE ; CORYNEBACTERIUM-GLUTAMICUM ; MICROSCALE THERMOPHORESIS ; BRADYRHIZOBIUM-JAPONICUM ; EVOLUTIONARY DYNAMICS ; GLOBAL REGULATOR |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000380346200014 |
| 资助机构 | National Basic Research Program of China Grant(2015CB755700) ; National Science Foundation of China(31230004 ; National High-Tech Program Grant(2012AA02A703) ; Institut Pasteur ; One Hundred-Talent Plan of the Chinese Academy of Sciences ; 31470205 ; 31470174) |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41490]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Lin, Min |
| 作者单位 | 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China 3.Inst Pasteur, F-75724 Paris 15, France |
| 推荐引用方式 GB/T 7714 | Zhan, Yuhua,Yan, Yongliang,Deng, Zhiping,et al. The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016,113(30):E4348-E4356. |
| APA | Zhan, Yuhua.,Yan, Yongliang.,Deng, Zhiping.,Chen, Ming.,Lu, Wei.,...&Lin, Min.(2016).The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,113(30),E4348-E4356. |
| MLA | Zhan, Yuhua,et al."The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113.30(2016):E4348-E4356. |
入库方式： OAI收割
来源：烟台海岸带研究所
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