Multi-omics reveal the molecular mechanism promoting the seed germination of Podophyllum hexandrum
文献类型：期刊论文
| 作者 | Ning, Rongchun; Fan, Tingting; Xu, Wenhua |
| 刊名 | INDUSTRIAL CROPS AND PRODUCTS
 |
| 出版日期 | 2025 |
| 卷号 | 234 |
| 关键词 | Podophyllum hexandrum Orthogonal experiment Plant growth regulator Rapid germination Plant hormone Gene expression |
| 英文摘要 | Podophyllum hexandrum is an endangered medicinal plant which seeds exhibit strong dormancy, posing challenges in natural and artificial propagation due to low germination rates, long time lags, and uneven germination. Building upon previous research, we conducted an orthogonal experiment with three factors and five levels, combining cold stratification and plant growth regulator treatment, to promote the germination of P. hexandrum seeds. The results indicated that under the treatment conditions of Group 23 in the gibberellic acid treatment, the germination rate reached as high as 96.7 %, with a germination time lag of 9 days. To elucidate the molecular mechanism, we repeated these conditions and collected 10 samples at 9-day intervals starting from day 0 for transcriptome and metabolome analysis (Five experimental groups and five control groups). Through metabolome detection, a total of 59 metabolites were identified. Transcriptome analysis revealed 78,827 differential genes, closely related to the synthesis, regulation, and transduction of plant hormones at different germination stages. Combined analysis identified 23 co-expressed metabolic pathways. Weighted Gene Co-expression Network Analysis (WGCNA) identified red and yellow-green modules related to the regulation of plant hormones of interest and pinpointed hub genes: Cluster-114934.0 and Cluster-114733.0. In this systematic study, we comprehensively screened technical methods to promote the germination of P. hexandrum seeds and deeply analyzed the molecular mechanism underlying this treatment-induced germination. Our findings provide a solid theoretical foundation and technical support for the artificial propagation of P. hexandrum. |
| 源URL | [http://210.75.249.4/handle/363003/62339]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Ning, Rongchun,Fan, Tingting,Xu, Wenhua. Multi-omics reveal the molecular mechanism promoting the seed germination of Podophyllum hexandrum[J]. INDUSTRIAL CROPS AND PRODUCTS,2025,234. |
| APA | Ning, Rongchun,Fan, Tingting,&Xu, Wenhua.(2025).Multi-omics reveal the molecular mechanism promoting the seed germination of Podophyllum hexandrum.INDUSTRIAL CROPS AND PRODUCTS,234. |
| MLA | Ning, Rongchun,et al."Multi-omics reveal the molecular mechanism promoting the seed germination of Podophyllum hexandrum".INDUSTRIAL CROPS AND PRODUCTS 234(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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