Effect of 7-O-glycosyl acylation of flavonoids from barley seedlings on α-glucosidase inhibition
文献类型：期刊论文
| 作者 | Chen, Tao; Lin, Changdong; Lu, Weihang; Wang, Shuo; Shen, Cheng; Luo, Juyuan; Li, Yulin |
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2025 |
| 卷号 | 488 |
| 关键词 | Flavonoids Acylation & Acy -Glucosidase Structure-activity relationship Inhibition mechanisms Multispectral study Molecular docking |
| 英文摘要 | In the present study, six 7-O-glycosyl acylation of flavonoids derived from lutonarin and saponarin were obtained from barley seedlings by medium-pressure LC, high-speed counter-current chromatography and preparative HPLC guided by UPLC-QTOF-MS/MS. Their inhibitory effects on alpha-glucosidase were investigated. Structure-activity relationship showed 7-O-glycosyl acylation enhanced inhibitory effect on alpha-glucosidase with coumaroyl > feruloyl > hydroxybenzoyl > sinapoyl. The IC50 values for saponarin and its four acylated products were 802.4 +/- 0.48 mu g/mL, 374.6 +/- 0.16 mu g/mL, 461.6 +/- 0.27 mu g/mL, 523.8 +/- 0.24 mu g/mL and 585.8 +/- 0.42 mu g/mL, respectively. Steady-state fluorescence spectroscopy revealed 7-O-glycosyl acylation enhanced the fluorescence quenching rate of alpha-glucosidase. Molecular docking revealed 7-O-glycosyl acylation formed more hydrogen bonds and hydrophobic interactions with alpha-glucosidase. Synchronous fluorescence spectroscopy showed all compounds altered the hydrophobic microenvironment surrounding the tryptophan residues in alpha-glucosidase. Circular dichroism spectroscopy indicated 7-O-glycosyl acylation modified the conformation and secondary structure of alpha-glucosidase. |
| 源URL | [http://210.75.249.4/handle/363003/62350]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Lin, Changdong,Lu, Weihang,et al. Effect of 7-O-glycosyl acylation of flavonoids from barley seedlings on α-glucosidase inhibition[J]. FOOD CHEMISTRY,2025,488. |
| APA | Chen, Tao.,Lin, Changdong.,Lu, Weihang.,Wang, Shuo.,Shen, Cheng.,...&Li, Yulin.(2025).Effect of 7-O-glycosyl acylation of flavonoids from barley seedlings on α-glucosidase inhibition.FOOD CHEMISTRY,488. |
| MLA | Chen, Tao,et al."Effect of 7-O-glycosyl acylation of flavonoids from barley seedlings on α-glucosidase inhibition".FOOD CHEMISTRY 488(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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