Satellite DNA in Populus and Molecular Karyotyping of Populus xiaohei and Its Derived Double Haploids
文献类型：期刊论文
| 作者 | Liu, Bo; Wang, Xinyu; Shen, Wenjie; Wang, Meng; Qu, Guanzheng; Dou, Quanwen |
| 刊名 | PLANTS-BASEL
 |
| 出版日期 | 2025 |
| 卷号 | 14期号:19 |
| 关键词 | P. xiaohei doubled haploid satDNA molecular karyotype chromosome variation |
| 英文摘要 | Karyotype analysis and the investigation of chromosomal variations in Populus are challenging due to its small and morphologically similar chromosomes. Despite its utility in chromosome identification and karyotype evolutionary research, satellite DNA (satDNA) remains underutilized in Populus. In the present study, 12 satDNAs were identified from P. trichocarpa, and the copy numbers and chromosomal distributions of each satDNA were analyzed bioinformatically in the reference genomes of P. trichocarpa, P. simonii, and P. nigra. Ten satDNA probes for fluorescence in situ hybridization (FISH) were successfully developed and validated on chromosomes of P. xiaohei (poplar hybrid P. simonii x P. nigra). By integrating bioinformatic genomic satDNA distribution patterns with experimental FISH signals, we constructed a molecular karyotype of P. xiaohei. Comparative analysis revealed errors in current poplar genome assemblies. Comparative karyotype analysis of P. xiaohei and its doubled haploid (DH) lines revealed chromosomal variations in the DH lines relative to the donor tree. The results demonstrate that the newly developed satDNA probes constitute robust cytogenetic tools for detecting structural variations in Populus, while molecular karyotyping provides new insights into the genetic mechanisms underlying chromosome variations in P. xiaohei and the DH plants derived. |
| 源URL | [http://210.75.249.4/handle/363003/62351]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Bo,Wang, Xinyu,Shen, Wenjie,et al. Satellite DNA in Populus and Molecular Karyotyping of Populus xiaohei and Its Derived Double Haploids[J]. PLANTS-BASEL,2025,14(19). |
| APA | Liu, Bo,Wang, Xinyu,Shen, Wenjie,Wang, Meng,Qu, Guanzheng,&Dou, Quanwen.(2025).Satellite DNA in Populus and Molecular Karyotyping of Populus xiaohei and Its Derived Double Haploids.PLANTS-BASEL,14(19). |
| MLA | Liu, Bo,et al."Satellite DNA in Populus and Molecular Karyotyping of Populus xiaohei and Its Derived Double Haploids".PLANTS-BASEL 14.19(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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