Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6
文献类型：期刊论文
| 作者 | Qin, Yuanyuan; Miyoshi, Ko; Yinsheng, Zhuoma; Fujiwara, Yuuki; Yoshimura, Takeshi; Katayama, Taiichi |
| 刊名 | CELLULAR SIGNALLING
 |
| 出版日期 | 2025 |
| 卷号 | 135 |
| 关键词 | AP-1 gamma 1-Adaptin Primary cilia Serotonin receptor type 6 G protein-coupled receptors |
| 英文摘要 | The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. In yeast, the interaction of this region with the C-terminal region of gamma 1-Adaptin, a subunit of adaptor protein complex 1 (AP-1), was identified. The interaction between Htr6 and gamma 1-Adaptin was confirmed by immunoprecipitation analysis using HEK293T cells. The preference for ciliary localization of Htr6 and Htr6 i4 was significantly decreased by ablation of gamma 1-Adaptin in hTERT RPE-1 cells, which was rescued by exogenous expression of gamma 1-Adaptin. Furthermore, Htr6 and Htr6 i4 showed reduced localization to primary cilia in gamma 1-Adaptin-depleted cultured hippocampal neurons compared with control neurons. These results indicate that the ciliary localization of Htr6 is facilitated by AP-1-mediated membrane trafficking. |
| 源URL | [http://210.75.249.4/handle/363003/62399]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Qin, Yuanyuan,Miyoshi, Ko,Yinsheng, Zhuoma,et al. Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6[J]. CELLULAR SIGNALLING,2025,135. |
| APA | Qin, Yuanyuan,Miyoshi, Ko,Yinsheng, Zhuoma,Fujiwara, Yuuki,Yoshimura, Takeshi,&Katayama, Taiichi.(2025).Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6.CELLULAR SIGNALLING,135. |
| MLA | Qin, Yuanyuan,et al."Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6".CELLULAR SIGNALLING 135(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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