Sulfonated polyetheretherketone enriched with MXene V2C promotes bone formation via WNT/β-catenin signaling in bone marrow mesenchymal stem cells
文献类型：期刊论文
| 作者 | Wang, Shengjie; Liu, Wei; Yang, Chao; Zhang, Xianlong; Lyu, Chunming |
| 刊名 | MEDICAL ENGINEERING & PHYSICS
 |
| 出版日期 | 2025 |
| 卷号 | 141 |
| 关键词 | SPEEK MXene V2C Osteogenesis MXene V2C rBMSCs WNT/beta-catenin Osteogenesis |
| 英文摘要 | Prosthetic loosening represents a catastrophic postoperative complication in artificial joint replacement, resulting in severe patient morbidity and substantial healthcare costs. This investigation aimed to develop a novel strategy for preventing prosthetic loosening. Two-dimensional MXene V2C nanosheets were synthesized and subsequently immobilized onto three-dimensional porous sulfonated polyetheretherketone (SPEEK) surfaces with polydopamine (PDA) to form V2C-PDA@SPEEK (Abbrev. V2C-PS). The biocompatibility was systematically evaluated using rat bone marrow mesenchymal stem cells (rBMSCs) and murine models. The osteogenic differentiation potential of V2C-PS was assessed through real-time PCR analysis, Alkaline phosphatase (ALP) staining, and Alizarin Red staining. The in vivo osteogenic capacity of V2C-PS surrounding the implant material was evaluated in rat femoral models using micro-CT analysis, biomechanical pull-out testing, sequential fluorescent labeling of newly formed bone, and Van Gieson staining. The molecular mechanisms underlying V2C-PS -mediated osteogenic differentiation of rBMSCs were investigated both in vitro and in vivo using chemical inhibitors, beta-catenin shRNA lentiviral silencing, and beta-catenin mRNA lentiviral overexpression. The results demonstrated that V2C-PS exhibited excellent biocompatibility. Quantitative analysis revealed substantial upregulation (p < 0.05) of critical osteogenic markers, including RUNX2 (4.4-fold), COL-1 (5.7-fold), OCN (3.3-fold), BMP-2 (4.4-fold), OPN (3.1-fold), BSP (3.3-fold), ON (4.3-fold), and OSX (2.83-fold), in the 25V(2)C-PS treatment group compared to PS group. The bone parameters were also remarkably enhanced in the 25V(2)C-PS group (BMD increased by 241 %, bone-implant contact by 159 %, BV/TV by 225 %, Tb.N by 281 %, Tb.Th by 214 %, maximum pull-out force by 250 %, and Tp.Sp decreased by 33 %). Furthermore, V2C-PS were found to enhance rBMSC osteogenic differentiation through activation of the Wnt/beta-catenin signaling pathway. This study presents a promising approach for preventing orthopedic prosthetic loosening and demonstrates significant potential for clinical translation. |
| 源URL | [http://210.75.249.4/handle/363003/62414]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Shengjie,Liu, Wei,Yang, Chao,et al. Sulfonated polyetheretherketone enriched with MXene V2C promotes bone formation via WNT/β-catenin signaling in bone marrow mesenchymal stem cells[J]. MEDICAL ENGINEERING & PHYSICS,2025,141. |
| APA | Wang, Shengjie,Liu, Wei,Yang, Chao,Zhang, Xianlong,&Lyu, Chunming.(2025).Sulfonated polyetheretherketone enriched with MXene V2C promotes bone formation via WNT/β-catenin signaling in bone marrow mesenchymal stem cells.MEDICAL ENGINEERING & PHYSICS,141. |
| MLA | Wang, Shengjie,et al."Sulfonated polyetheretherketone enriched with MXene V2C promotes bone formation via WNT/β-catenin signaling in bone marrow mesenchymal stem cells".MEDICAL ENGINEERING & PHYSICS 141(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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