Optimization of Fermentation Conditions for the Production of 2,3,5-Trimethylpyrazine by Recombinant Bacillus licheniformis
文献类型：期刊论文
| 作者 | Liu, Xun; Gu, Hongyi; Wang, Handong; Tang, Zhen; Chen, Shuanglian; Li, Han; Quan, Wenli |
| 刊名 | MICROORGANISMS
 |
| 出版日期 | 2025 |
| 卷号 | 13期号:7 |
| 关键词 | 2,3,5-trimethylpyrazine Bacillus licheniformis BlTDH gene recombinant strains fermentation optimization |
| 英文摘要 | 2,3,5-Trimethylpyrazine (TMP) is an alkyl pyrazine with broad application prospects in the fields of food additives and medicine. L-threonine-3-dehydrogenase (TDH) is a key enzyme in the biosynthesis pathway of TMP. To explore the efficient and environmentally friendly production method of TMP, we constructed recombinant strains overexpressing the BlTDH gene and its mutant BlTDH (N157A) using Bacillus licheniformis YC7. The TMP yield of recombinant strains with pHT01-BlTDH (N157A) reached 15.35 +/- 1.51 mg/L, which was significantly higher than that of strains with pHT01-BlTDH (9.86 +/- 1.24 mg/L) and strains with vector pHT01 (2.35 +/- 0.58 mg/L). To further increase the TMP yield of strain YC7/pHT01-BlTDH (N157A), the fermentation process was optimized by single-factor experiments, and the response surface test was conducted using the Box-Behnken design. The results revealed that the substrate ratio, IPTG concentration and fermentation time had significant effects on the yield of TMP, and the degree of influence was substrate ratio > fermentation time > IPTG concentration. The optimization results of response surface indicated that the optimal fermentation conditions were as follows: substrate ratio of 1:2, IPTG concentration of 1.0 mM, and fermentation time of 4 d. Under these conditions, the TMP yield reached 44.52 +/- 0.21 mg/L, which was around 0.005 mg/L different from the predicted value (45.515 mg/L), and increased by 29.17 mg/L compared with the initial condition. The optimization of fermentation conditions significantly increased the yield of TMP produced by recombinant strains, which provided a theoretical basis and strain resources for industrial production of TMP. |
| 源URL | [http://210.75.249.4/handle/363003/62416]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Xun,Gu, Hongyi,Wang, Handong,et al. Optimization of Fermentation Conditions for the Production of 2,3,5-Trimethylpyrazine by Recombinant Bacillus licheniformis[J]. MICROORGANISMS,2025,13(7). |
| APA | Liu, Xun.,Gu, Hongyi.,Wang, Handong.,Tang, Zhen.,Chen, Shuanglian.,...&Quan, Wenli.(2025).Optimization of Fermentation Conditions for the Production of 2,3,5-Trimethylpyrazine by Recombinant Bacillus licheniformis.MICROORGANISMS,13(7). |
| MLA | Liu, Xun,et al."Optimization of Fermentation Conditions for the Production of 2,3,5-Trimethylpyrazine by Recombinant Bacillus licheniformis".MICROORGANISMS 13.7(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。