Development of a semi-dwarf dominant genic male sterile wheat with seed red fluorescence as a visible marker
文献类型：期刊论文
| 作者 | Zheng, Ke; Zhou, Xiaonan; Xu, Ningyao; Liu, Demei; Wang, Haiqing |
| 刊名 | MOLECULAR BREEDING
 |
| 出版日期 | 2025 |
| 卷号 | 45期号:6 |
| 关键词 | Wheat Dominant male sterility Semi-dwarf Red fluorescence protein Visible marker |
| 英文摘要 | Taigu genic male-sterile wheat, containing the dominant gene male sterile (Ms) 2, shows completely male-sterility in different genetic background and under various environments. The development of Aibai wheat with tightly linked Ms2 and Reduced height (Rht)-D1c, offers possibility for identifying the male-sterile plants by investigation of reduced plant height. However, due to the extreme dwarfism of Aibai wheat caused by Rht-D1c, it inhabits a shadowed microenvironment caused by its fertile sibling plants. This results in delayed maturation of sterile plant progeny. In order to develop a novel germplasm with new visible marker for sorting male sterile plants conferred by Ms2, a binary vector containing Bar, Ms2, Rht-D1b, and DsRed driven by the aleurone-specific promoter Ltp2 was constructed and introduced into the wheat Fielder using Agrobacterium-mediated transformation in this study. After investigation of the fertility, plant height and seed fluorescence of positive transgenic wheat plants, a line exhibited semi-dwarf male sterility, which could be reliably identified by the aleurone-specifically expressed red fluorescence in seeds, serving as a genetically stable reporter. Therefore, this study provides a novel male-sterile wheat that offers a powerful tool for hybrid seed production and facilitates genetic improvement in wheat through recurrent selection. |
| 源URL | [http://210.75.249.4/handle/363003/62434]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zheng, Ke,Zhou, Xiaonan,Xu, Ningyao,et al. Development of a semi-dwarf dominant genic male sterile wheat with seed red fluorescence as a visible marker[J]. MOLECULAR BREEDING,2025,45(6). |
| APA | Zheng, Ke,Zhou, Xiaonan,Xu, Ningyao,Liu, Demei,&Wang, Haiqing.(2025).Development of a semi-dwarf dominant genic male sterile wheat with seed red fluorescence as a visible marker.MOLECULAR BREEDING,45(6). |
| MLA | Zheng, Ke,et al."Development of a semi-dwarf dominant genic male sterile wheat with seed red fluorescence as a visible marker".MOLECULAR BREEDING 45.6(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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