Screening of hepatoprotective natural products from Floccularia luteovirens via a novel HPLC-hepatocyte protein system combined with oil red O quantification strategy
文献类型：期刊论文
| 作者 | Liu, Chuang; Lin, Zikai; Tong, Yingying; Wang, Qilan; Li, Gang; Dang, Jun |
| 刊名 | MICROCHEMICAL JOURNAL
 |
| 出版日期 | 2025 |
| 卷号 | 213 |
| 关键词 | Floccularia luteovirens HPLC-hepatocyte protein system Dipeptides FAS ADMET |
| 英文摘要 | The efficient recognition of bioactive components in natural products (NPs) relies on the innovation and development of various methodologies. To identify the hepatoprotective secondary metabolites in Floccularia luteovirens (F. luteovirens), a novel HPLC-hepatocyte protein (HPLC-HP) system was established for the qualitative identification of active peaks interacting with hepatocyte proteins in F. luteovirens fractions at the chromatographic level, using the protein solution from a liver injury cell model as the mobile phase. By combining this system with oil red O quantification for activity screening of the fractions after each preparation, ultimately leading to the successful isolation of three novel dipeptides, named flolutsin A-C. Subsequent in vitro experiments and computational simulations validated the reliability of the HPLC-HP system, these three novel dipeptides effectively reduced oleic acid-induced damage in HepG2 cells through fatty acid synthase. Additionally, ADMET analysis was conducted to assess their pharmaceutical values. In conclusion, this study further clarified the pharmacological basis of the hepatoprotective activity of F. luteovirens, while the novel HPLC-HP system developed in this study demonstrated strong application potential, and such an integrated strategy provides a practical approach for screening bioactive components from other NPs. |
| 源URL | [http://210.75.249.4/handle/363003/62455]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Chuang,Lin, Zikai,Tong, Yingying,et al. Screening of hepatoprotective natural products from Floccularia luteovirens via a novel HPLC-hepatocyte protein system combined with oil red O quantification strategy[J]. MICROCHEMICAL JOURNAL,2025,213. |
| APA | Liu, Chuang,Lin, Zikai,Tong, Yingying,Wang, Qilan,Li, Gang,&Dang, Jun.(2025).Screening of hepatoprotective natural products from Floccularia luteovirens via a novel HPLC-hepatocyte protein system combined with oil red O quantification strategy.MICROCHEMICAL JOURNAL,213. |
| MLA | Liu, Chuang,et al."Screening of hepatoprotective natural products from Floccularia luteovirens via a novel HPLC-hepatocyte protein system combined with oil red O quantification strategy".MICROCHEMICAL JOURNAL 213(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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