Development of a highly sensitive C1QC-targeting aptamer and its application in a modified antibody-aptamer sandwich biosensor for C1QC biomarker detection
文献类型：期刊论文
| 作者 | Wu, Wenwei; Qian, Lixia; Shen, Jie; Li, Hongmei; Li, Liang-cheng |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2025 |
| 卷号 | 439 |
| 关键词 | C1QC aptamer Magnetic beads-SELEX Enzyme-linked antibody-aptamer sandwich (ELAAS) biosensor Chemiluminescent antibody-aptamer sandwich (CLAAS) biosensor C1QC cancer biomarker detection |
| 英文摘要 | Cancer remains a leading cause of mortality worldwide, with China bearing a particularly high burden. Early detection through reliable biomarkers is essential for improving patient outcomes. This study reports the development and validation of a highly specific aptamer-based biosensor for detecting the complement protein C1QC, a promising cancer biomarker. Using a multi-step selection process involving Magnetic beads-SELEX, qPCR, flow cytometry, high-throughput sequencing, surface plasmon resonance (SPR), pull-down, and western blotting, we identified Apt 13, a slow-dissociation aptamer with high specificity and affinity for C1QC (KD = 48.7 nM). SPR confirmed the formation of a sandwich complex between Apt 13, C1QC, and Anti_C1QC, confirming their distinct binding sites. We developed two biosensor platforms based on this aptamer: the enzymelinked antibody-aptamer sandwich (ELAAS) and chemiluminescent antibody-aptamer sandwich (CLAAS) biosensors. The ELAAS biosensor, designed for visual colorimetric detection, demonstrated a limit of detection (LOD) of 98 ng/mL. In contrast, the CLAAS system, utilizing acridinium ester-modified antibodies, achieved an enhanced LOD of 0.375 ng/mL. Receiver Operating Characteristic (ROC) analysis on serum samples from 75 hepatocellular carcinoma (HCC) patients and 20 healthy controls yielded an AUC of 0.942, with 86.67 % sensitivity and 95.00 % specificity, demonstrating CLAAS's high diagnostic accuracy in differentiating HCC from controls. Further testing in HCC patient serum samples revealed significantly elevated C1QC levels compared to healthy controls, underscoring C1QC's potential as a HCC cancer biomarker. Apt 13 presents a promising tool for the development of innovative, sensitive, and cost-effective diagnostic technologies for C1QC detection and may offer future potential for targeted therapies. |
| 源URL | [http://210.75.249.4/handle/363003/62463]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wu, Wenwei,Qian, Lixia,Shen, Jie,et al. Development of a highly sensitive C1QC-targeting aptamer and its application in a modified antibody-aptamer sandwich biosensor for C1QC biomarker detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2025,439. |
| APA | Wu, Wenwei,Qian, Lixia,Shen, Jie,Li, Hongmei,&Li, Liang-cheng.(2025).Development of a highly sensitive C1QC-targeting aptamer and its application in a modified antibody-aptamer sandwich biosensor for C1QC biomarker detection.SENSORS AND ACTUATORS B-CHEMICAL,439. |
| MLA | Wu, Wenwei,et al."Development of a highly sensitive C1QC-targeting aptamer and its application in a modified antibody-aptamer sandwich biosensor for C1QC biomarker detection".SENSORS AND ACTUATORS B-CHEMICAL 439(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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