Structural characterization and protective activity of α-glucans derived from Cynomorium songaricum Rupr. against homocysteine-induced oxidative stress in MC3T3-E1 cells
文献类型：期刊论文
| 作者 | Li, Mingcong; Wang, Wenshu; Li, Guoqiang; Gong, Baihui; Wang, Qiannan; Bi, Hongtao; Zhou, Yubi |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2025 |
| 卷号 | 310 |
| 关键词 | Cynomorium songaricum Rupr. Keap1/Nrf2 signalling pathway Osteoporosis |
| 英文摘要 | Cynomorium songaricum Rupr. is a traditional Chinese medicine renowned for its ability to nourish the kidneys and enhance bone marrow. However, the composition and pharmacological actions of its primary component, polysaccharides, are not fully understood. In this study, three alpha-glucans (CSRP-50, CSRP-70, and CSRP-90) were isolated from C. songaricum, and their backbones are identical, consisting of repeating units of 1,4-linked alpha-DGlcp and 1,6-linked alpha-D-Glcp. However, CSRP-50 is devoid of side chains, while CSRP-70 and CSRP-90 feature side chains consisting of 1,6-linked alpha-D-Glcp and terminal 1-linked alpha(beta)-D-Glcp, which are attached at the O-6 position of the 1,4-linked alpha-D-Glcp in the backbones. Pharmacological studies demonstrated that these three glucans have significant protective effects against homocysteine-induced oxidative stress damage in MC3T3-E1 cells, as indicated by their increased cellular vitality and the levels of glutathione, superoxide dismutase, and total antioxidant capacity, while reducing malondialdehyde and lactate dehydrogenase levels. Further studies have shown that these three glucans can significantly reduce Keap1 expression, while increasing Nrf2, HO-1, and NQO1 expression, and have certain free radical scavenging ability in vitro, suggesting their protective activity via the activation of the Keap1/Nrf2 signalling pathway and directly eliminating free radicals from homocysteineinduced oxidative stress damage in MC3T3-E1 cells. |
| 源URL | [http://210.75.249.4/handle/363003/62467]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Li, Mingcong,Wang, Wenshu,Li, Guoqiang,et al. Structural characterization and protective activity of α-glucans derived from Cynomorium songaricum Rupr. against homocysteine-induced oxidative stress in MC3T3-E1 cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2025,310. |
| APA | Li, Mingcong.,Wang, Wenshu.,Li, Guoqiang.,Gong, Baihui.,Wang, Qiannan.,...&Zhou, Yubi.(2025).Structural characterization and protective activity of α-glucans derived from Cynomorium songaricum Rupr. against homocysteine-induced oxidative stress in MC3T3-E1 cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,310. |
| MLA | Li, Mingcong,et al."Structural characterization and protective activity of α-glucans derived from Cynomorium songaricum Rupr. against homocysteine-induced oxidative stress in MC3T3-E1 cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 310(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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