High-fat diet alters retinal lipid composition and gene expression networks in mice
文献类型：期刊论文
| 作者 | Zou, Rong; Cai, Jinrui; Chen, Tianyu; Mo, Wenhui; Qian, Hao; Zhu, Xianjun; Zhang, Lin |
| 刊名 | BMC BIOLOGY
 |
| 出版日期 | 2025 |
| 卷号 | 23期号:1 |
| 关键词 | HFD Metabolic homeostasis Retina Cholesterol |
| 英文摘要 | BackgroundHigh-fat diet (HFD) was suggested to be associated with several retinal diseases, including age-related macular degeneration (AMD), glaucoma, and diabetic retinopathy (DR). Nevertheless, our understanding of the mechanisms governing retinal lipid metabolic homeostasis remains limited, with little attention focused on the influence of HFD on different retinal cell types. To address this gap, we established a high-fat model using mice fed with HFD for a duration of 6 months. Then, we conducted a comparative analysis of the retinal lipidome and proteome between normal diet (ND) and HFD-fed mice to explore the impacts of HFD on retinal lipid metabolism and gene expression network. Furthermore, we also investigated the impacts of HFD on retina in single-cell resolution by single-cell transcriptome sequencing.ResultsWe found that a long-term HFD significantly altered the lipid composition of the retina, with a dramatically elevated cholesterylesters (CE), phosphatidylcholine (PC), and phosphatidylglycerol (PG) level and a decreased eicosanoid level. Proteomic analysis revealed that the primary bile acid biosynthesis pathway was over-activated in HFD retinas. By using single-cell transcriptome analysis, we identified different regulation of gene expression in MG and rod cells in a high-fat environment, whereas the previously identified activation of the bile acid synthesis pathway was predominantly found in MG cells, and may be regulated by alternative pathways of bile acid synthesis, suggesting the critical roles of MG cells in retinal lipid metabolism.ConclusionsTaken together, by multi-omics studies, we unveiled that HFD leading to the development of retinal diseases may be regulated by alternative pathways of bile acid synthesis, and our study will shed light on the treatment of these diseases. |
| 源URL | [http://210.75.249.4/handle/363003/62470]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zou, Rong,Cai, Jinrui,Chen, Tianyu,et al. High-fat diet alters retinal lipid composition and gene expression networks in mice[J]. BMC BIOLOGY,2025,23(1). |
| APA | Zou, Rong.,Cai, Jinrui.,Chen, Tianyu.,Mo, Wenhui.,Qian, Hao.,...&Zhang, Lin.(2025).High-fat diet alters retinal lipid composition and gene expression networks in mice.BMC BIOLOGY,23(1). |
| MLA | Zou, Rong,et al."High-fat diet alters retinal lipid composition and gene expression networks in mice".BMC BIOLOGY 23.1(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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