Investigation on the Inhibitory Effect of Bergenia purpurascens Water Extract on COVID-19 XBB1.5 Variant and Its Blocking ACE2 Mechanism
文献类型：期刊论文
| 作者 | Shi, Yongxia; Sun, Xiaoning; Yu, Zhaoxia; Dai, Dalan; Guo, Hongyu; Mei, Lijuan; Dai, Jun; Jiang, Lei |
| 刊名 | NATURAL PRODUCT COMMUNICATIONS
 |
| 出版日期 | 2025 |
| 卷号 | 20期号:4 |
| 关键词 | Non-specific ACE2 inhibitor COVID-19 XBB 1.5 Bergenia purpurascens UPLC-MS/MS molecular docking |
| 英文摘要 | Objective Bergenia purpurascens, a medicinal herb indigenous to southwestern China, has garnered interest for its potential therapeutic properties. This study investigates the herb's water extract's capacity to interfere with the interaction between the COVID-19 S1 Receptor Binding Domain (RBD) and the ACE2 receptor, a critical step in the virus's infection process.Methods Quality standards for the Bergenia purpurascens extract were established to ensure consistency. The extract's efficacy was assessed using a Vero-E6 cell infection model to inhibit live virus infection of the COVID-19 XBB1.5 variant. Surface Plasmon Resonance (SPR) was employed to explore the mechanism of action. The extract's composition was further analyzed using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS), with active compounds identified through a combination of in silico screening and actual activity testing.Results The water extract of Bergenia purpurascens demonstrated significant inhibitory activity against the COVID-19 XBB1.5 variant, with stability and high potency, reflected by an IC50 value of 0.037 mg/mL. The selectivity index for the antiviral effect is approximately 9. The extract's composition was predominantly Phenylpropanoids and Carbohydrates, accounting for over 60% of the total. Interestingly, a majority of the high-content compounds were inactive, while the low-content compounds, exemplified by Ellagic acid, showed synergistic activity. The extract's inhibitory mechanism was characterized as a non-specific inhibition of ACE2 with a steady-state binding affinity (KD) of 7.9 x 10-7 M.Conclusions The study concludes that the water extract of Bergenia purpurascens is a promising, cost-effective non-specific ACE2 inhibitor with potential applications in COVID-19 therapy. The identification of active compounds and the elucidation of its inhibitory mechanism provide a foundation for further research and development of this traditional medicinal herb in combating viral infections. |
| 源URL | [http://210.75.249.4/handle/363003/62484]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Shi, Yongxia,Sun, Xiaoning,Yu, Zhaoxia,et al. Investigation on the Inhibitory Effect of Bergenia purpurascens Water Extract on COVID-19 XBB1.5 Variant and Its Blocking ACE2 Mechanism[J]. NATURAL PRODUCT COMMUNICATIONS,2025,20(4). |
| APA | Shi, Yongxia.,Sun, Xiaoning.,Yu, Zhaoxia.,Dai, Dalan.,Guo, Hongyu.,...&Jiang, Lei.(2025).Investigation on the Inhibitory Effect of Bergenia purpurascens Water Extract on COVID-19 XBB1.5 Variant and Its Blocking ACE2 Mechanism.NATURAL PRODUCT COMMUNICATIONS,20(4). |
| MLA | Shi, Yongxia,et al."Investigation on the Inhibitory Effect of Bergenia purpurascens Water Extract on COVID-19 XBB1.5 Variant and Its Blocking ACE2 Mechanism".NATURAL PRODUCT COMMUNICATIONS 20.4(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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