DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Zhang, Xiaojuan; Liu, Yao; Liu, Weiwei; Shen, Yuhu; Cao, Fangqi; Deng, Yunxia; Cao, Zhangjun; Huang, Zhiwei |
| 刊名 | JOURNAL OF BASIC MICROBIOLOGY
 |
| 出版日期 | 2025 |
| 卷号 | 65期号:5 |
| 关键词 | dgk1p Gemfibrozil lipid metabolism transcription factors yeast |
| 英文摘要 | Gemfibrozil (GEM) is a phenoxy aromatic acid-based lipid-lowering drug. It activates peroxisome proliferator-activated receptor alpha (PPAR-alpha), which leads to altered lipid metabolism and lowers serum triglyceride levels by modulating lipoprotein lipase. However, the action of the mode of GEM is still unclear. Herein, the model organism Saccharomyces cerevisiae was applied to explore the molecular mechanism of GEM regulating lipid metabolism. The results showed that the triacylglycerol (TAG) content and the number of lipid droplets of yeast increased significantly after GEM treatment in the wild-type BY4741. Screening of mutations related to lipid metabolism pathways (PAH1, DGK1, TGL3, TGL4, LRO1, ARE1, ARE2, and DGA1) showed that dgk1 Delta had no change in lipid accumulation under GEM. In the wild type, GEM inhibited the expression of DGK1, resulting in a significant decrease in the contents of phospholipids (phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS)) and neutral lipids (TAG and diacylglycerol (DAG)). However, their abundances could not be changed in dgk1 Delta after the treatment with GEM Luciferase assay further showed that DGK1 may be a target of GEM to induce lipid accumulation via TUP1/CYC8, which could act on the DGK1 promoter-TATA highly conserved element (-400 bp - 200 bp). Altogether, the effect of GEM on lipid metabolism was associated with the upregulation of TUP1/CYC8, leading to a decrease in the expression of DGK1, thereby increasing the TAG content in yeast cells. It is expected that the data will help to clarify the molecular mechanism of GEM regulating lipid metabolism in humans. |
| 源URL | [http://210.75.249.4/handle/363003/62529]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaojuan,Liu, Yao,Liu, Weiwei,et al. DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae[J]. JOURNAL OF BASIC MICROBIOLOGY,2025,65(5). |
| APA | Zhang, Xiaojuan.,Liu, Yao.,Liu, Weiwei.,Shen, Yuhu.,Cao, Fangqi.,...&Huang, Zhiwei.(2025).DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae.JOURNAL OF BASIC MICROBIOLOGY,65(5). |
| MLA | Zhang, Xiaojuan,et al."DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae".JOURNAL OF BASIC MICROBIOLOGY 65.5(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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