CRISPR/Cas9-mediated knockout of PvTCP19/22 enhances tiller number and biomass yield in switchgrass
文献类型：期刊论文
| 作者 | Sun, Ying; Yang, Ruijuan; Liu, Min; Liu, Yuchen; Yuan, Xiaoyi; Chen, Longhao; Zhao, Siyi; Qin, Xiaochun; Zhou, Chuanen; Fu, Chunxiang |
| 刊名 | INDUSTRIAL CROPS AND PRODUCTS
 |
| 出版日期 | 2025 |
| 卷号 | 226 |
| 关键词 | Switchgrass PvTCP19/22 Gene editing Tiller number Biomass yield |
| 英文摘要 | Controlling tiller number is a promising strategy to improve biomass yield for switchgrass, which is an important lignocellulosic energy crop used for biofuel production. Therefore, the functions of PvTCP19 and its paralog PvTCP22, two members of the TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) family, were investigated for increasing tiller number and biomass yield. These data revealed that PvTCP19 localizes in the nucleus and is highly expressed in leaf organ. Using a Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR Associated 9 (CRISPR/Cas9) system to knock out PvTCP19, both PvTCP19 and PvTCP22 were edited efficiently in most of the positive transgenic lines (50/53) and the tetra-allelic homozygous editing efficiency was 80 % (40/50). The knockout mutants demonstrated significant improvements: tiller number increased, dry biomass yield rose by 92 %, and soluble sugar content increased by 120 % compared with those of control plants. Additionally, the mutants exhibited superior nitrogen and phosphorus use efficiencies. Transcriptome analysis revealed that PvTCP19/22 likely repress the expression of DWARFAND LOW-TILLERING (PvDLT) and activate the transcription of DORMANCY-ASSOCIATED (PvDRM) genes, which are key regulators of tiller outgrowth and bud dormancy. These findings establish PvTCP19/22 as potential pivotal inhibitors of tillering downstream of the strigolactone (SL) signaling pathway. Overall, this study identified PvTCP19/22 as valuable targets for CRISPR/Cas9-based genetic enhancement of switchgrass, paving the way for increased biomass yield and sugar production. |
| 源URL | [http://210.75.249.4/handle/363003/62531]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Ying,Yang, Ruijuan,Liu, Min,et al. CRISPR/Cas9-mediated knockout of PvTCP19/22 enhances tiller number and biomass yield in switchgrass[J]. INDUSTRIAL CROPS AND PRODUCTS,2025,226. |
| APA | Sun, Ying.,Yang, Ruijuan.,Liu, Min.,Liu, Yuchen.,Yuan, Xiaoyi.,...&Liu, Wenwen.(2025).CRISPR/Cas9-mediated knockout of PvTCP19/22 enhances tiller number and biomass yield in switchgrass.INDUSTRIAL CROPS AND PRODUCTS,226. |
| MLA | Sun, Ying,et al."CRISPR/Cas9-mediated knockout of PvTCP19/22 enhances tiller number and biomass yield in switchgrass".INDUSTRIAL CROPS AND PRODUCTS 226(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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