Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping
文献类型：期刊论文
| 作者 | Wang, Guowen; Shu, Shi; Fu, Changqi; Huang, Rong; Xu, Shangrong; Zhang, Jun; Peng, Wei |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2025 |
| 卷号 | 26期号:4 |
| 关键词 | PRDM9 yak protein interaction meiotic recombination hybrid sterility |
| 英文摘要 | Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks during spermatogenesis. PRDM9 determines the localization of recombination hotspots by interacting with several protein complexes in mammals. The function of PRDM9 is not well understood during spermatogenesis in mice or yaks. In this study, we applied yeast two-hybrid assays combined with next-generation sequencing techniques to screen the complete set of PRDM9-interacting proteins and explore its novel functions in yak spermatogenesis. Our results showed that 267 PRDM9-interacting proteins were identified. The gene ontology (GO) analysis of the interacting proteins revealed that the GO terms were primarily associated with spermatogenesis, positive regulation of double-strand break repair via homologous recombination, RNA splicing, the ubiquitin-dependent ERAD pathway, and other biological processes. MKX and PDCD5 were verified to be strongly interacting with PRDM9 and expressed in prophase I of meiosis in both mouse and yak testes. The localizations of RNA splicing genes including THOC5, DDX5, and XRCC6 were expressed in spermatocytes. Cattleyak is the hybrid offspring of a yak and a domestic cow, and the male offspring are sterile. The gene expression of the interacting proteins was also examined in the sterile male hybrid of yak and cattle. Among the 58 detected genes, 55 were downregulated in cattleyak. In conclusion, we established a complete PRDM9 interaction network, and a novel function of PRDM9 was identified, which will further promote our understanding of spermatogenesis. It also provides new insights for the study of hybrid male sterility. |
| 源URL | [http://210.75.249.4/handle/363003/62545]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Guowen,Shu, Shi,Fu, Changqi,et al. Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2025,26(4). |
| APA | Wang, Guowen.,Shu, Shi.,Fu, Changqi.,Huang, Rong.,Xu, Shangrong.,...&Peng, Wei.(2025).Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,26(4). |
| MLA | Wang, Guowen,et al."Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26.4(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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