Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes
文献类型：期刊论文
| 作者 | Li, Hongmei; Chen, Tao; Shen, Cheng; Wang, Shuo; Luo, Juyuan; Song, Zhibo; Ma, Yumei; Li, Aijing; Lu, Weihang; Feng, Hailun |
| 刊名 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 出版日期 | 2025 |
| 卷号 | 63期号:2 |
| 英文摘要 | Separation of polar compounds especially with similar polarities is challenging. In the present study, three polar compounds with similar polarities, including gentiopicroside, sweroside and mangiferin, have been successfully separated from Swertia mussotii by a combination of two counter-current chromatography modes. With the selected solvent system of ethyl acetate/n-butanol/water (8/2/10, v/v), a continuous injection mode was firstly employed. As a result, 10.4 g of mangiferin with purity higher than 98% and 8 g of Peak I mainly composed of gentiopicroside and sweroside could be easily obtained from 24 g of the sample by counter-current chromatography through eight injections. Then, a recycling counter-current chromatography mode was introduced for the separation of gentiopicroside and sweroside. Through six cycles, 5.3 g of gentiopicroside and 0.8 g of sweroside with purity higher than 98% were obtained from 8 g of Peak I. The research provides a reference for the large-scale separation of polar compounds with similar polarities. |
| 源URL | [http://210.75.249.4/handle/363003/62556]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Li, Hongmei,Chen, Tao,Shen, Cheng,et al. Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2025,63(2). |
| APA | Li, Hongmei.,Chen, Tao.,Shen, Cheng.,Wang, Shuo.,Luo, Juyuan.,...&Li, Yulin.(2025).Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes.JOURNAL OF CHROMATOGRAPHIC SCIENCE,63(2). |
| MLA | Li, Hongmei,et al."Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes".JOURNAL OF CHROMATOGRAPHIC SCIENCE 63.2(2025). |
入库方式： OAI收割
来源：西北高原生物研究所
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