Enhanced stability of Thermus thermophiles SG0.5JP17-16 laccase immobilized by CuF-MOF and its application in the Congo red decolorization
文献类型：期刊论文
| 作者 | Sun, Hengsong1; Jiang, Fangming2; Yang, Xiaorong1 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2024-09-01 |
| 卷号 | 209页码:11 |
| 关键词 | Immobilization Encapsulation Surface adsorption Laccase CuF-MOF |
| ISSN号 | 1369-703X |
| DOI | 10.1016/j.bej.2024.109397 |
| 通讯作者 | Yang, Xiaorong(birxyang@scut.edu.cn) |
| 英文摘要 | Laccase attracts the extensive attention in degradation of pollutants due to its environmental friendliness and high efficiency. Free laccase has the disadvantages of easy inactivity, poor stability and difficulty in reuse, so its application in industry is confined. The immobilization of laccase can protect the laccase and actualize the cycle. In this work, copper fumarate metal-organic-frameworks (CuF-MOFs) were prepared and utilized to immobilize the Thermus thermophiles SG0.5JP17 - 16 laccase (lacTT) by encapsulation and surface adsorption at room temperature. The results from SEM, FTIR, XRD and BET indicated that lacTT was immobilized successfully. The optimal pH and temperature of lacTT@CuF-MOF and lacTT#CuF-MOF were determined to be pH 6.5 at 95 degrees C and pH 5.0 at 95 degrees C respectively. The immobilized laccase had the elevated stability in storage and reuse. The lacTT@CuF-MOF displayed higher thermal stability as compared with lacTT#CuF-MOF. But the V max (11.15 mu M. min -1 ) value of lacTT@CuF-MOF was lower than that of lacTT#CuF-MOF ( V max = 37.61 mu M.min - 1 ). The degradation efficiencies of lacTT@CuF-MOF and lacTT#CuF-MOF on Congo red were 41 % and 30 % at 6th cycle respectively. Therefore, the MOF was beneficial and could serve as sustainable carrier for laccase immobilization. The immobilized laccase might be more efficient to degrade the dyes in wastewater from textile industry than free enzyme. |
| WOS关键词 | METAL-ORGANIC FRAMEWORKS ; DEGRADATION ; ADSORPTION ; OXIDASES ; REMOVAL |
| 资助项目 | National Key R & D Program of China[2021YFC2102700] ; Guangdong Natural Science Foundation[2022A1515011415] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:001258933700001 |
| 出版者 | ELSEVIER |
| 资助机构 | National Key R & D Program of China ; Guangdong Natural Science Foundation |
| 源URL | [http://ir.giec.ac.cn/handle/344007/42255]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Yang, Xiaorong |
| 作者单位 | 1.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Hengsong,Jiang, Fangming,Yang, Xiaorong. Enhanced stability of Thermus thermophiles SG0.5JP17-16 laccase immobilized by CuF-MOF and its application in the Congo red decolorization[J]. BIOCHEMICAL ENGINEERING JOURNAL,2024,209:11. |
| APA | Sun, Hengsong,Jiang, Fangming,&Yang, Xiaorong.(2024).Enhanced stability of Thermus thermophiles SG0.5JP17-16 laccase immobilized by CuF-MOF and its application in the Congo red decolorization.BIOCHEMICAL ENGINEERING JOURNAL,209,11. |
| MLA | Sun, Hengsong,et al."Enhanced stability of Thermus thermophiles SG0.5JP17-16 laccase immobilized by CuF-MOF and its application in the Congo red decolorization".BIOCHEMICAL ENGINEERING JOURNAL 209(2024):11. |
入库方式： OAI收割
来源：广州能源研究所
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