Aminated lignin improved enzymatic hydrolysis of cellulosic substrate treated by p-toluenesulfonic acid
文献类型：期刊论文
| 作者 | Yu, Chunyang1,2; Wang, Zekang2; Fu, Xiangjin1; Liu, Chun1; Li, Anping1; Lin, Qinlu1; Lan, Tianqing1,2; Zhuang, Xinshu3,4,5 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2024-11-20 |
| 卷号 | 395页码:44-52 |
| 关键词 | Enzymatic hydrolysis Aminated lignin Cellulase activities Interaction force Molecular docking |
| ISSN号 | 0168-1656 |
| DOI | 10.1016/j.jbiotec.2024.09.004 |
| 通讯作者 | Lan, Tianqing(lantianqing_8019@163.com) |
| 英文摘要 | Lignin can affect the enzymatic hydrolysis efficiency of lignocellulose. In this study, the lignin isolated from sugarcane bagasse (SCB) pretreated with p-toluenesulfonic acid (PL) was firstly aminated, and then the effects of PL and aminated PL (APL) on the bagasse enzymatic hydrolysis efficiency (EHE) were investigated. The results showed that the addition of PL and APL promoted the EHE, and EHE with APL (73.82 %) was higher than PL (51.39 %). To explore the reason, the data were further analyzed including cellulase adsorption capacity, enzyme activity, cellulase-lignin interaction, and molecular docking. It was found that APL adsorbed more cellulase (27.83 mg protein/g lignin) than PL (4.96 mg protein/g lignin), resulting from the greater interaction force and lower binding free energy between APL and cellulase. The addition of APL more remarkably enhanced the cellobiohydrolase and endoglucanase activities than PL due to more effectively inducing cellulase conformation optimization. |
| WOS关键词 | SACCHARIFICATION ; INHIBITION ; BINDING |
| 资助项目 | Hunan Provincial Natural Science Foundation of China[2023JJ30985] ; National Natural Science Foundation of China[22168020] ; Interna-tional Science and Technology Innovation Cooperation Program of the National Key Research and Development Plan of China[2021YFE0114400] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001322523500001 |
| 出版者 | ELSEVIER |
| 资助机构 | Hunan Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; Interna-tional Science and Technology Innovation Cooperation Program of the National Key Research and Development Plan of China |
| 源URL | [http://ir.giec.ac.cn/handle/344007/42999]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Lan, Tianqing |
| 作者单位 | 1.Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Res Ctr Rice & Byproduct Deep Proc, 498 South Shaoshan Rd, Changsha 410004, Peoples R China 2.Kunming Univ Sci & Technol, Fac Food Sci & Engn, 727 South Jingming Rd, Kunming 650500, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, 2 Energy Rd, Guangzhou 510640, Peoples R China 4.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 5.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Energy Rd, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Chunyang,Wang, Zekang,Fu, Xiangjin,et al. Aminated lignin improved enzymatic hydrolysis of cellulosic substrate treated by p-toluenesulfonic acid[J]. JOURNAL OF BIOTECHNOLOGY,2024,395:44-52. |
| APA | Yu, Chunyang.,Wang, Zekang.,Fu, Xiangjin.,Liu, Chun.,Li, Anping.,...&Zhuang, Xinshu.(2024).Aminated lignin improved enzymatic hydrolysis of cellulosic substrate treated by p-toluenesulfonic acid.JOURNAL OF BIOTECHNOLOGY,395,44-52. |
| MLA | Yu, Chunyang,et al."Aminated lignin improved enzymatic hydrolysis of cellulosic substrate treated by p-toluenesulfonic acid".JOURNAL OF BIOTECHNOLOGY 395(2024):44-52. |
入库方式： OAI收割
来源：广州能源研究所
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