Effect of cellulase-assisted cold isostatic pressure extraction on the characteristics and functional properties of polyphenol extracts from camellia sinensis seeds
文献类型：期刊论文
| 作者 | Sang, Xueting1; Zhen, Feng2; Li, Hongru1; Zhang, Zhiyun3; Wang, Yuxin1; Qu, Bin1; Sun, Yongming2 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2024-12-01 |
| 卷号 | 282页码:10 |
| 关键词 | Cold isostatic pressure Camellia sinensis seed Polyphenol |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2024.137384 |
| 通讯作者 | Qu, Bin(qubin@neau.edu.cn) |
| 英文摘要 | In this experiment, polyphenolic substances were extracted from Camellia sinensis seeds (CSS) using a synergistic treatment of cold isostatic pressure (CIP) and cellulase. The effects of pressure, treatment time, and cellulase addition on the experiment were investigated. And the optimal extraction conditions were established by single factor experiment and Box-benhken experiments: the pressure applied by CIP was 408.649 MPa, the treatment time was 10.995 min, and the cellulase addition was 4.098 %. The polyphenols in the extract were characterized and quantified using LC-MS/MS. By comparing the different treatments, it was found that the synergistic treatment of CIP and cellulase resulted in a higher extraction yield. FTIR, XRD and SEM mapping showed that CIP synergistic pretreatment with cellulase was able to disrupt the microstructure of the plant and promote the influx of the active ingredients into solution. Finally, the activity of the extracts was detected by using in vitro antioxidant experiments and RAW264.7 cellular anti-inflammatory experiments, which indicated that CIP and cellulase synergistically treated polyphenol extracts had high antioxidant and anti-inflammatory capacity. This experiment provides a new pretreatment method for extracting active substances from CSS. |
| WOS关键词 | OIL ; QUANTIFICATION ; ACID |
| 资助项目 | National Natural Science Foundation of China[32102725] ; Taiyuan Donglong Machinery Co., Ltd. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001357311200001 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; Taiyuan Donglong Machinery Co., Ltd. |
| 源URL | [http://ir.giec.ac.cn/handle/344007/43559]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Qu, Bin |
| 作者单位 | 1.Northeast Agr Univ, Coll Art & Sci, Harbin 150030, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 3.Northeast Agr Univ, Coll Anim Med, Harbin 150030, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sang, Xueting,Zhen, Feng,Li, Hongru,et al. Effect of cellulase-assisted cold isostatic pressure extraction on the characteristics and functional properties of polyphenol extracts from camellia sinensis seeds[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,282:10. |
| APA | Sang, Xueting.,Zhen, Feng.,Li, Hongru.,Zhang, Zhiyun.,Wang, Yuxin.,...&Sun, Yongming.(2024).Effect of cellulase-assisted cold isostatic pressure extraction on the characteristics and functional properties of polyphenol extracts from camellia sinensis seeds.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,282,10. |
| MLA | Sang, Xueting,et al."Effect of cellulase-assisted cold isostatic pressure extraction on the characteristics and functional properties of polyphenol extracts from camellia sinensis seeds".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 282(2024):10. |
入库方式： OAI收割
来源：广州能源研究所
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