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  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Detection of Exosomal MicroRNA with Hybrid Plasmonic Nanoheater-Driven PCR for Pulmonary Fibrosis Progression

文献类型：期刊论文

作者Zhang, Yu1,2; Li, Jin1,2; Wu, Yixuan1,5; Wang, Yunqing1; Wang, Xiaoyan3; Lv, Changjun2; Chen, Lingxin1,4,5
刊名SMALL
出版日期2025-11-17
页码14
关键词bronchoalveolar lavage fluid exosomal microRNA hybrid plasmonic nanoheaters photothermal-driven PCR pulmonary fibrosis
ISSN号1613-6810
DOI10.1002/smll.202510264
通讯作者Li, Jin(jinli@yic.ac.cn) ; Lv, Changjun(lucky_lcj@bzmc.edu.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Pulmonary fibrosis (PF) is a severe respiratory disease, but early diagnosis of PF is challenging. Here, a non-invasive approach is developed to rapidly detect bronchoalveolar lavage fluid (BALF)-derived exosomal microRNAs (miRNAs) for PF progression based on hybrid nanoheater-driven digital polymerase chain reaction (nanodPCR). The hybrid nanoheaters, composed of plasmonic nanostructures and attached black hole quenchers (BHQs), generate a synergistic photothermal effect from the plasmonic field amplification. Gelatin microcarriers doped with such nanoheaters enable ultra-rapid thermal cycling (9.5 degrees C s-1 heating rate) under near-infrared laser irradiation, which is sufficient for ultrafast thermal cycling. This rapid thermal cycling capability, coupled with fluorescence detection, allows precise quantification of exosomal miRNAs through nanodPCR, thereby facilitating longitudinal monitoring of PF progression with an ultrashort turnover time (8.8 min), nearly seven-fold faster than conventional PCR protocols (approximate to 60 min). Compared with normal mice, expression of miR-142-3p and miR-21-5p in PF mice at different stages significantly increases over time, while let-7d-5p expression decreases. Furthermore, clinical validation in 40 PF patients through this nanodPCR platform identifies disease-stage-specific miRNA profiles from healthy controls. Therefore, this work not only presents a robust photothermal-driven PCR platform but also demonstrates the potential of BALF-derived exosomal miRNA as non-invasive biomarkers for PF progression.
WOS关键词EXTRACELLULAR VESICLES ; ACTIVATION ; SECRETION
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种英语
WOS记录号WOS:001614950500001
资助机构National Natural Science Foundation of China
源URL[http://ir.yic.ac.cn/handle/133337/41615]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Li, Jin; Lv, Changjun; Chen, Lingxin
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zone Ecol Environm Monitoring Technol & Eq, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
2.Binzhou Med Univ, Binzhou Med Univ Hosp, Dept Resp & Crit Care Med, Binzhou 256603, Peoples R China
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
5.Shaoxing Univ, Inst Adv Study, Shaoxing 312000, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Yu,Li, Jin,Wu, Yixuan,et al. Detection of Exosomal MicroRNA with Hybrid Plasmonic Nanoheater-Driven PCR for Pulmonary Fibrosis Progression[J]. SMALL,2025:14.
APA Zhang, Yu.,Li, Jin.,Wu, Yixuan.,Wang, Yunqing.,Wang, Xiaoyan.,...&Chen, Lingxin.(2025).Detection of Exosomal MicroRNA with Hybrid Plasmonic Nanoheater-Driven PCR for Pulmonary Fibrosis Progression.SMALL,14.
MLA Zhang, Yu,et al."Detection of Exosomal MicroRNA with Hybrid Plasmonic Nanoheater-Driven PCR for Pulmonary Fibrosis Progression".SMALL (2025):14.

入库方式： OAI收割

来源：烟台海岸带研究所

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