Comparative transcriptomic and metabolomic analyses reveal different tolerance mechanisms to heat stress in two geographic populations of Argopecten irradians concentricus
文献类型：期刊论文
| 作者 | Shen, Min4,6; Wu, Shaoxuan3,5; Liu, Oscar Yichen1; Wang, Jinjing3,5; Liu, Guilong2; Xu, Xin2; Liu, Zhigang4; Deng, Yuewen4; Ning, Junhao3,5; Wang, Chunde3,5 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2026-02-01 |
| 卷号 | 613页码:14 |
| 关键词 | Argopecten irradians concentricus Heat stress Transcriptomic Metabolomic Haemocyte Enzymatic activity |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2025.743404 |
| 通讯作者 | Ning, Junhao(jhning@yic.ac.cn) ; Wang, Chunde(chundewang2007@163.com) |
| 英文摘要 | Temperature significantly affects the survival, growth, and physiological functions of marine organisms. The bay scallop southern subspecies, Argopecten irradians concentricus, well known for its high temperature tolerance, has not been deeply explored in terms of the molecular regulatory mechanism underlying its heat stress tolerance trait. In this study, we investigated the mechanisms underlying heat stress tolerance in two geographically distinct populations of A. i. concentricus, the southern population (SP) from Zhanjiang in southern China and the northern population (NP), which was originally transplanted from Zhanjiang and reared for 4 generations in Yantai waters. The upper incipient lethal temperature (LT50) of SP (31.81 +/- 0.14 degrees C) was significantly higher than that of NP (29.25 +/- 0.06 degrees C), indicating a significant decline in heat tolerance in NP following its transplantation and breeding in the northern waters. Comparative transcriptomic and metabolomic analyses, along with physiological measurements, revealed distinct regulatory mechanisms in two populations. NP predominantly up-regulated energy and metabolic pathways, including carbohydrate, amino acid, and lipid metabolism, whereas SP enhanced the expression in immune-related pathways such as NF-kappa B, FoxO, AMPK, and MAPK signaling. However, the cGMP-PKG signaling pathway, the calcium signaling pathway, and the C-type lectin receptor signaling pathway were down-regulated in both the NP and SP groups. Metabolomic profiling revealed that SP exhibited more diversified metabolic regulations than the NP populations. Weighted Gene Co-expression Network Analysis (WGCNA) identified key genes strongly associated with heat stress, including GPX1, CYP2B5, and PLA2G4A within the magenta module, all of which are involved in the arachidonic acid pathway. Integrated co-analysis further pinpointed biomarkers and genes linked to heat stress adaptation. Physiological analyses demonstrated that SP possessed stronger antioxidant defenses than NP, as evidenced by higher activities of antioxidant enzymes (CAT, POD, SOD) and lower MDA levels. Additionally, SP displayed more robust immune responses, including higher haemocyte counts and phagocytosis rates, and lower apoptosis rates, to heat stress. In conclusion, our study comprehensively reveals the different tolerance mechanisms of the two A. i. concentricus populations to heat stress. The findings may enhance our understanding of the heat tolerance mechanisms in scallops, providing new insights for optimizing aquaculture practices, such as selective breeding and environmental management. |
| WOS关键词 | CELL-CYCLE ARREST ; MYTILUS-GALLOPROVINCIALIS ; SEAWATER TEMPERATURE ; LIPID-PEROXIDATION ; CRASSOSTREA-GIGAS ; OXIDATIVE STRESS ; IMMUNE ; RESPONSES ; EXPRESSION ; GILLS |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001619348000003 |
| 资助机构 | Breeding and Industrialization of Improved Bay Scallop ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System ; Science and Technology Innovation Capacity Improvement Project of Shandong Province |
| 源URL | [http://ir.yic.ac.cn/handle/133337/41658]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Ning, Junhao; Wang, Chunde |
| 作者单位 | 1.St Georges Sr Sch, Vancouver, BC V6S 1V1, Canada 2.Yantai Spring Sea AquaSeed Co Ltd, Yantai 264006, Peoples R China 3.Yantai Key Lab Scallop Breeding, Yantai 264003, Shandong, Peoples R China 4.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China 6.Shandong Marine Resources & Environm Res Inst, Engn & Res Ctr Marine Fishery Feed Shandong Prov, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Min,Wu, Shaoxuan,Liu, Oscar Yichen,et al. Comparative transcriptomic and metabolomic analyses reveal different tolerance mechanisms to heat stress in two geographic populations of Argopecten irradians concentricus[J]. AQUACULTURE,2026,613:14. |
| APA | Shen, Min.,Wu, Shaoxuan.,Liu, Oscar Yichen.,Wang, Jinjing.,Liu, Guilong.,...&Wang, Chunde.(2026).Comparative transcriptomic and metabolomic analyses reveal different tolerance mechanisms to heat stress in two geographic populations of Argopecten irradians concentricus.AQUACULTURE,613,14. |
| MLA | Shen, Min,et al."Comparative transcriptomic and metabolomic analyses reveal different tolerance mechanisms to heat stress in two geographic populations of Argopecten irradians concentricus".AQUACULTURE 613(2026):14. |
入库方式： OAI收割
来源：烟台海岸带研究所
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