A solid-state membrane potentiometric microsensor for in situ sensing of NH4+in soybean root nodules
文献类型：期刊论文
| 作者 | Wu, Yinlong1,2; Yang, Yanxu1,2; Ding, Jiawang1,2 |
| 刊名 | TALANTA
 |
| 出版日期 | 2026-05-01 |
| 卷号 | 301页码:8 |
| 关键词 | Potentiometric microelectrode Solid-state membrane Prussian blue analogue Root nodules Biological nitrogen fixation |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2025.129321 |
| 通讯作者 | Ding, Jiawang(jwding@yic.ac.cn) |
| 英文摘要 | Soybean-rhizobia symbiotic nitrogen fixation, a process in which rhizobia mediate biological nitrogen fixation by converting inert atmospheric nitrogen (N2) into biologically available forms (e.g., ammonium, NH4+), has been extensively investigated. However, non-invasive, in situ monitoring methods for this process remain lacking. Herein, we report a solid-state membrane potentiometric ammonium ion-selective microelectrode (NH4+-IS mu E) for the in situ detection of NH4+ in soybean root nodules. A Prussian blue analogue with ion channels, which enables the specific insertion/extraction of NH4+ ions while excluding interfering cations, was electrodeposited on a carbon fiber to fabricate the microelectrode. The cation sorption capability and ion selectivity of the thin film were explored by modulating the intercalation/deintercalation process and reducing the interfering cations within the framework. The NH4+-IS mu E exhibits a Nernstian response to NH4+ over the concentration range of 1.0 x 10-6 to 1.0 x 10-3 M, with a detection limit of 6.2 x 10-7M. This sensor enables in situ, real-time detection of NH4+-the direct product of biological nitrogen fixation in the legume plant-rhizobium symbiotic system. The release of NH4+ ions in soybean root nodules during nitrogen fixation was successfully monitored. Overall, this work provides a simple and versatile tool for studying and monitoring biological symbiotic nitrogen fixation processes. |
| WOS关键词 | ION-SELECTIVE ELECTRODES ; MICROELECTRODE ; COEFFICIENTS ; COMPOSITE ; SENSORS ; COPPER |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001658303200001 |
| 资助机构 | National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Natural Science Foundation of Shandong Province ; Special Fund for the Scholar Program of Yantai ; Special Fund for the Scholar Program of Shandong Province |
| 源URL | [http://ir.yic.ac.cn/handle/133337/42007]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Ding, Jiawang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Zone Environm Proc & Ecol, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Yinlong,Yang, Yanxu,Ding, Jiawang. A solid-state membrane potentiometric microsensor for in situ sensing of NH4+in soybean root nodules[J]. TALANTA,2026,301:8. |
| APA | Wu, Yinlong,Yang, Yanxu,&Ding, Jiawang.(2026).A solid-state membrane potentiometric microsensor for in situ sensing of NH4+in soybean root nodules.TALANTA,301,8. |
| MLA | Wu, Yinlong,et al."A solid-state membrane potentiometric microsensor for in situ sensing of NH4+in soybean root nodules".TALANTA 301(2026):8. |
入库方式： OAI收割
来源：烟台海岸带研究所
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