Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645
文献类型：期刊论文
| 作者 | Pu, Yang3; Liu, Chuanxu3; Lan, Xiangyu3; Xiao, Chao1; Xie, Mingyuan5; Li, Wenjun2; Chen, Min4 |
| 刊名 | JOURNAL OF FLUORESCENCE
 |
| 出版日期 | 2026-02-09 |
| 页码 | 12 |
| 关键词 | Cryptophyte phycocyanin 645 Natural photosynthetic antennae Hydrophobic interaction chromatography Time-resolved fluorescence spectroscopy Energy transfer kinetic process |
| ISSN号 | 1053-0509 |
| DOI | 10.1007/s10895-025-04663-7 |
| 通讯作者 | Xie, Mingyuan(xiemy5@mail.sysu.edu.cn) ; Li, Wenjun(wjli@yic.ac.cn) ; Chen, Min(chenmclm@163.com) |
| 英文摘要 | As a remarkable model protein for studying excitation energy transfer (EET), Cryptophyte phycocyanin 645 (Cr-PC645) is not only important in the frontier research field on photosynthesis but also, like other kinds of phycocyanin, valuable in food and cosmetic industries as a kind of natural blue pigment protein. Here, we report a convenient preparation method for the purification of Cr-PC645 from cryptophytic algae, Chroomonas placoidea, mainly using hydrophobic interaction chromatography, and a study of EET characterization based on it. The final recovery of Cr-PC645 is 58.49% and the highest purity of final samples reaches 7.27 (A(645)/A(280)) in the obtained products. In addition to high purity, the properties of unchanged intact optical activity of purified Cr-PC645 are proved by comprehensively analyzing absorption, fluorescence, circular dichroism, and ultrafast time-resolved spectra. Furthermore, three time components of 6, 59, and 1021 ps are confirmed by 77 K time-resolved fluorescence spectroscopy experiments, which can be assigned to the time of energy transfer process between specific phycocyanobilins (PCBs), suggesting that the excitation energy is transferred from PCB-158 to PCB-82 in the same beta subunit and localized the PCB-82 chromophores between different beta subunits, then transferred to the downstream acceptors. In view of all these results, this optimized method is efficient and will be conducive to multi-field applications in the future. |
| WOS关键词 | LIGHT-HARVESTING PROTEIN ; CRYSTAL-STRUCTURE ; TRANSFER DYNAMICS ; PHYCOERYTHRIN ; HEXAMER ; ALGAE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001685649500001 |
| 资助机构 | The National Key Research and Development Program of China ; Shandong provincial education system government sponsored study abroad program and Ludong University research initiation funds |
| 源URL | [http://ir.yic.ac.cn/handle/133337/42098]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Xie, Mingyuan; Li, Wenjun; Chen, Min |
| 作者单位 | 1.Sun Yat sen Univ, Sch Life Sci, State Key Lab Biocontrol,MOE Key Lab Gene Funct &, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Ludong Univ, Sch Fisheries, Yantai 264025, Peoples R China 4.Yantai Univ, Inst Life Sci, Yantai 264005, Peoples R China 5.Sun Yat sen Univ, Sch Phys, Guangzhou 510275, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Yang,Liu, Chuanxu,Lan, Xiangyu,et al. Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645[J]. JOURNAL OF FLUORESCENCE,2026:12. |
| APA | Pu, Yang.,Liu, Chuanxu.,Lan, Xiangyu.,Xiao, Chao.,Xie, Mingyuan.,...&Chen, Min.(2026).Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645.JOURNAL OF FLUORESCENCE,12. |
| MLA | Pu, Yang,et al."Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645".JOURNAL OF FLUORESCENCE (2026):12. |
入库方式： OAI收割
来源：烟台海岸带研究所
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