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  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Magnetic mesoporous silica nanocarriers integrated aptamer-based colorimetric biosensor enables sensitive detection of P. aeruginosa

文献类型：期刊论文

作者Sun, Kaidi1; Zhang, Yan1; Zheng, Qingyin1; Qin, Dan1; Yan, Peng1; Wang, Liyan4; Chen, Hao3; Chen, Lingxin1,2; Bi, Liyan1
刊名MICROCHEMICAL JOURNAL
出版日期2026-04-01
卷号223页码:9
关键词Magnetic mesoporous silica nanocarriers Aptamer HRP Colorimetric biosensor P. aeruginosa
ISSN号0026-265X
DOI10.1016/j.microc.2026.117354
通讯作者Chen, Hao(haochen@ytu.edu.cn) ; Chen, Lingxin(lxchen@yic.ac.cn) ; Bi, Liyan(liyan_bi@bzmc.edu.cn)
英文摘要P. aeruginosa, a highly adaptable and drug-resistant Gram-negative bacterium, poses a serious threat to public healthcare. We developed a magnetic mesoporous silica nanocarriers integrated aptamer-based colorimetric biosensor (MMNCS) for P. aeruginosa rapid and sensitive detection. In the biosensor, magnetic mesoporous silica nanoparticles served as carriers for the horseradish peroxidase (HRP), with aptamers acting as specific capture probes to target bacteria. In the presence of P. aeruginosa, the biosensor anchored onto the bacterial surface, leading to pore closure and suppression of TMB oxidation by HRP. The established colorimetric biosensor achieved remarkable performance in a broad range (10 to 105 CFU/mL), high sensitivity (limit of detection, 0.8 CFU/mL) and rapid assay time (15 min). Furthermore, the MMNCS platform was evaluated in tap water, artificial urine, artificial saliva and human serum samples, exhibiting good recoveries of 95.47% to 105.65% and a relative standard deviation between 0.93% and 4.17%. The approach provides a low-cost, sensitive and rapid platform for early screening detection of pathogenic bacteria.
WOS关键词MICROSPHERES ; TEMPERATURE
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001693971900001
资助机构Key R & D Program of Shandong Province, China ; Shandong Science and Technology Foundation ; National Natural Science Foundation of China ; Special Funding for the Case-by-Case Introduction of Top Talent (Teams) Program in Yantai
源URL[http://ir.yic.ac.cn/handle/133337/42164]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Chen, Hao; Chen, Lingxin; Bi, Liyan
作者单位1.Binzhou Med Univ, Sch Special Educ & Rehabil, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res,Coastal Zone Ecol Env, Shandong Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Yantai Univ, Sch Environm & Mat Engn, Yantai 264005, Peoples R China
4.Shandong Technol & Business Univ, Sch Management Sci & Engn, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Kaidi,Zhang, Yan,Zheng, Qingyin,et al. Magnetic mesoporous silica nanocarriers integrated aptamer-based colorimetric biosensor enables sensitive detection of P. aeruginosa[J]. MICROCHEMICAL JOURNAL,2026,223:9.
APA Sun, Kaidi.,Zhang, Yan.,Zheng, Qingyin.,Qin, Dan.,Yan, Peng.,...&Bi, Liyan.(2026).Magnetic mesoporous silica nanocarriers integrated aptamer-based colorimetric biosensor enables sensitive detection of P. aeruginosa.MICROCHEMICAL JOURNAL,223,9.
MLA Sun, Kaidi,et al."Magnetic mesoporous silica nanocarriers integrated aptamer-based colorimetric biosensor enables sensitive detection of P. aeruginosa".MICROCHEMICAL JOURNAL 223(2026):9.

入库方式： OAI收割

来源：烟台海岸带研究所

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