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UuPGRP, a peptidoglycan recognition protein of Urechis unicinctus, promotes immune defense against bacterial infection

文献类型：期刊论文

作者Qiu, Yulie1,2,4; Shi, Chenxi1,2,4; Li, Xupeng1,2,4; Jiao, Xudong3; Zhang, Jian1,2,4
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2026-05-01
卷号172页码:12
关键词Urechis unicinctus Peptidoglycan recognition protein Reactive oxygen species Innate immunity
ISSN号1050-4648
DOI10.1016/j.fsi.2026.111210
通讯作者Jiao, Xudong(xdjiao@yic.ac.cn) ; Zhang, Jian(zhangjian@ytu.edu.cn)
英文摘要Peptidoglycan recognition proteins (PGRPs) are evolutionarily conserved innate immune receptors with pivotal roles in regulating host defense responses, yet the functional characterization of PGRPs in marine benthic invertebrates, especially in Echiurioidea and Annelida, remains limited. In this study, we identified and characterized a short-type PGRP from the U. unicinctus, designated UuPGRP, to elucidate its role in antimicrobial immunity. Sequence analysis revealed that UuPGRP consists of 232 amino acid residues, including a conserved Cterminal PGRP domain. Phylogenetic and sequence identity analyses demonstrated that UuPGRP shares 40.72%- 59.09% sequence identity with PGRPs from other species. Tissue distribution profiling in healthy U. unicinctus showed widespread expression of UuPGRP across multiple tissues, with the highest expression level in anal sac. Notably, following infection with the Vibrio anguillarum, UuPGRP exhibited significant tissue-specific transcriptional regulation. Functional analysis revealed that rUuPGRP interacts with a broad range of Gram-positive and -negative bacteria in a dose-dependent manner. Moreover, rUuPGRP mediated Zn2+-dependent bacterial agglutination, which can be abrogated by EDTA. Importantly, rUuPGRP enhanced reactive oxygen species (ROS) production in hemocytes, suggesting its ability to modulate oxidative burst. Finally, in vivo clearance assays showed that rUuPGRP significantly accelerated the elimination of V. anguillarum from infected U. unicinctus, directly confirming its contribution to pathogen clearance. Collectively, these findings demonstrate that UuPGRP functions as an important immune effector, mediating bacterial recognition and amplifying immune responses via ROS modulation and agglutination. This work expands our understanding of PGRP-mediated immunity in non-model organisms, shedding light on their adaptive strategies for survival in microbe-rich benthic environments.
WOS关键词MESSENGER-RNA EXPRESSION ; INNATE IMMUNITY ; MOLECULAR-CLONING ; IDENTIFICATION ; PGRP ; INVOLVEMENT ; TOLL ; ZEBRAFISH ; PATHWAYS ; DOMAIN
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:001694256000001
资助机构Cultivation Project for Leading Enterprises in Aquatic Seed Industry of Shandong ; Improvement of Aquatic Seed Industry Project of Yantai ; Double Hundred Plan Blue Industry Leading Talent Project of Yantai ; Academy of Finland (AKA)
源URL[http://ir.yic.ac.cn/handle/133337/42167]  
专题海岸带生物学与生物资源利用重点实验室
通讯作者Jiao, Xudong; Zhang, Jian
作者单位1.Yantai Univ, Sch Ocean, Yantai, Peoples R China
2.Shandong Engn Res Ctr Hlth Land Sea Relay Farming, Yantai, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Peoples R China
4.Yantai Engn Res Ctr Deep Sea Aquaculture Econ Fish, Yantai, Peoples R China
推荐引用方式
GB/T 7714		 Qiu, Yulie,Shi, Chenxi,Li, Xupeng,et al. UuPGRP, a peptidoglycan recognition protein of Urechis unicinctus, promotes immune defense against bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2026,172:12.
APA Qiu, Yulie,Shi, Chenxi,Li, Xupeng,Jiao, Xudong,&Zhang, Jian.(2026).UuPGRP, a peptidoglycan recognition protein of Urechis unicinctus, promotes immune defense against bacterial infection.FISH & SHELLFISH IMMUNOLOGY,172,12.
MLA Qiu, Yulie,et al."UuPGRP, a peptidoglycan recognition protein of Urechis unicinctus, promotes immune defense against bacterial infection".FISH & SHELLFISH IMMUNOLOGY 172(2026):12.

入库方式： OAI收割

来源：烟台海岸带研究所

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