Transcriptome-Metabolome Integration Uncovers Salt Stress Effects on Flavonoid Biosynthesis in Two Self-Selected Breeding Alfalfa Varieties
文献类型：期刊论文
| 作者 | Zhu, Xinqiang1; Yang, Yang1,2; Fu, Yunxing2; Leng, Feifan2; Li, Shaowei3; Wang, Yonggang2; Cao, Zhonghua4; Wang, Xiaoli1; Yang, Hongshan1; Wu, Fang1 |
| 刊名 | PHYSIOLOGIA PLANTARUM
 |
| 出版日期 | 2026-01-08 |
| 卷号 | 178期号:1页码:e70728 |
| 关键词 | alfalfa differential genes differential metabolites flavonoids biosynthesis salt stress |
| ISSN号 | 0031-9317 |
| DOI | 10.1111/ppl.70728 |
| 产权排序 | 3 |
| 文献子类 | Article |
| 英文摘要 | Salt stress constrains plant distribution and productivity, posing challenges to agriculture and ecosystems. Alfalfa (Medicago sativa L.) is one of the most important forages in the world. Seed germination, epigenetic physiology, transcriptome, metabolome, and the common regulatory mechanism of transcriptome and metabolome were investigated in Xizang's first independently bred alfalfa, ZangMu 1 (ZM1, highly salt-resistant type) and ZangMu 2 (ZM2, salt-sensitive type), under the treatments of 0, 100, and 200 mmol L-1 NaCl. The results showed that the salt tolerance of ZM1 was significantly better than that of ZM2. Additionally, the seed germination and physiological indices of both varieties exhibited a trend of low promotion and high inhibition. The joint transcriptome and metabolome analyses revealed that the flavonoid biosynthesis pathway was the core pathway in response to salt stress, and ZM1 enhanced stress tolerance by significantly upregulating more differential genes and metabolites. The levels of five key antioxidant metabolites (naringenin (NAR), apigenin (API), dihydroquercetin (DHQ), galangin (GAL), and epigallocatechin (EGC)) were significantly changed under salt stress, indicating that the free radical scavenging system of the plant was regulated. The expression levels of the core genes (CHI1, FL3H, CYP9B16, CYP75A1, FLS, and LAR) showed a synergistic regulation pattern with the salt tolerance metabolites, and the results of qRT-PCR validation were highly consistent with the transcriptome data. This study systematically analysed the flavonoid metabolic network of salt tolerance in Xizang alfalfa, providing molecular targets and a theoretical basis for the selection and breeding of salt-tolerant varieties. |
| URL标识 | 查看原文 |
| WOS关键词 | GROWTH ; ACCUMULATION ; SELECTIVITY ; TOLERANCE ; L. |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001656841200001 |
| 出版者 | WILEY |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/219634]  |
| 专题 | 生态系统网络观测与模拟院重点实验室_外文论文 |
| 通讯作者 | Wang, Xiaoli; Yang, Hongshan |
| 作者单位 | 1.Lanzhou Inst Husb & Pharmaceut Sci CAAS, Lanzhou, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China; 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing, Peoples R China; 4.Xizang Agr & Anim Husb Univ, Linzhi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Xinqiang,Yang, Yang,Fu, Yunxing,et al. Transcriptome-Metabolome Integration Uncovers Salt Stress Effects on Flavonoid Biosynthesis in Two Self-Selected Breeding Alfalfa Varieties[J]. PHYSIOLOGIA PLANTARUM,2026,178(1):e70728. |
| APA | Zhu, Xinqiang.,Yang, Yang.,Fu, Yunxing.,Leng, Feifan.,Li, Shaowei.,...&Duan, Huirong.(2026).Transcriptome-Metabolome Integration Uncovers Salt Stress Effects on Flavonoid Biosynthesis in Two Self-Selected Breeding Alfalfa Varieties.PHYSIOLOGIA PLANTARUM,178(1),e70728. |
| MLA | Zhu, Xinqiang,et al."Transcriptome-Metabolome Integration Uncovers Salt Stress Effects on Flavonoid Biosynthesis in Two Self-Selected Breeding Alfalfa Varieties".PHYSIOLOGIA PLANTARUM 178.1(2026):e70728. |
入库方式： OAI收割
来源：地理科学与资源研究所
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