Mechanism of Engineered Rhizobia Overexpressing Cytokinin Improve Heat Stress Response in Alfalfa
文献类型：期刊论文
| 作者 | Qi, Xiangyu1; Shen, Xiaoxia1; Luo, Li3; Huang, Shudi1; Yu, Wenzhe1; Li, Yanrong1; Liu, Huanhuan2; Yang, Peizhi1 |
| 刊名 | PLANT CELL AND ENVIRONMENT
 |
| 出版日期 | 2026-02-08 |
| 卷号 | N/A |
| 关键词 | alfalfa cytokinin engineered rhizobia heat stress transcription regulation |
| ISSN号 | 0140-7791 |
| DOI | 10.1111/pce.70396 |
| 产权排序 | 3 |
| 文献子类 | Article ; Early Access |
| 英文摘要 | Heat stress is a major abiotic stress that limits alfalfa (Medicago sativa L.) productivity. To test whether cytokinin-overexpressing engineered rhizobia could enhance heat tolerance, we inoculated alfalfa with modified rhizobia and evaluated their physiological and molecular responses. We found that inoculation with engineered rhizobia significantly increased trans-Zeatin content in alfalfa. Compared with control rhizobia-inoculated plants, engineered rhizobia-inoculated plants exhibited increased plant height, fresh/dry weight, relative water content, and photosynthetic efficiency (total chlorophyll and carotenoids), alongside reduced hydrogen peroxide (H2O2) and superoxide (O-2.-) levels under heat stress. RNA-seq analysis revealed that engineered rhizobia upregulated heat stress-responsive genes in alfalfa, which was further verified by qRT-PCR. Metabolomics analyses showed significant alterations in phenylpropanoid, flavonoid, phenolic acid, and salicylic acid metabolic pathways in engineered rhizobia-inoculated plants under heat stress. Contrary to conventional approaches, our results demonstrate that cytokinin-overexpressing rhizobia not only enhance alfalfa heat tolerance but also activate multi-pathway stress responses. Collectively, these findings propose a novel strategy for developing heat-tolerant alfalfa through engineered rhizobia-mediated cytokinin biosynthesis, which helps to promote the development of sustainable alfalfa breeding for heat-tolerant varieties. |
| URL标识 | 查看原文 |
| WOS关键词 | SINORHIZOBIUM-MELILOTI ; GENE-EXPRESSION ; SALT STRESS ; TOLERANCE ; DROUGHT ; PLANTS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001684463500001 |
| 出版者 | WILEY |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/220905]  |
| 专题 | 生态系统网络观测与模拟院重点实验室_外文论文 |
| 通讯作者 | Yang, Peizhi |
| 作者单位 | 1.Northwest A&F Univ, Coll Grassland Agr, Yangling, Shaanxi, Peoples R China; 2.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing, Peoples R China 3.Shanghai Univ, Synthet Biol Res Ctr, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Qi, Xiangyu,Shen, Xiaoxia,Luo, Li,et al. Mechanism of Engineered Rhizobia Overexpressing Cytokinin Improve Heat Stress Response in Alfalfa[J]. PLANT CELL AND ENVIRONMENT,2026,N/A. |
| APA | Qi, Xiangyu.,Shen, Xiaoxia.,Luo, Li.,Huang, Shudi.,Yu, Wenzhe.,...&Yang, Peizhi.(2026).Mechanism of Engineered Rhizobia Overexpressing Cytokinin Improve Heat Stress Response in Alfalfa.PLANT CELL AND ENVIRONMENT,N/A. |
| MLA | Qi, Xiangyu,et al."Mechanism of Engineered Rhizobia Overexpressing Cytokinin Improve Heat Stress Response in Alfalfa".PLANT CELL AND ENVIRONMENT N/A(2026). |
入库方式： OAI收割
来源：地理科学与资源研究所
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