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Main controls on phoD-harboring bacterial community abundance, composition, and activity from oil-contaminated soils at the Changqing oilfield, Northwest China

文献类型：期刊论文

作者Li, Dandan1,3,4,6; Du, Xianyuan6; Li, Xingchun6; Zhang, Xinyu4,5; Zheng, Jin6; Chu, Qin3; Qiu, Weiwen2; Zhang, Hanyu6; Wang, Qingwei6
刊名FRONTIERS IN MICROBIOLOGY
出版日期2026-01-13
卷号16页码:1732638
关键词total petroleum hydrocarbons oil contamination alkaline phosphatase activity phoD-harboring bacteria microbial community Changqing oil field
DOI10.3389/fmicb.2025.1732638
产权排序2
文献子类Article
英文摘要Background: Soil phosphorus (p) availability limits the native microbial activity, which then inhibits the petroleum hydrocarbon biodegradation. Microbial communities harbouring the alkaline phosphatase (ALP) phoD gene (phoD-harboring bacteria community, hereafter) play the key roles in the regulation of P availability in soils. Nevertheless, the consequences of oil contamination on ALP activity and phoD-harboring bacterial community dynamics are poorly understood. It is necessary to assess phoD-harboring bacterial abundance, community diversity, and ALP activity in response to oil contamination. This information would be useful for formulating plans for future bioremediation processes. Methods: In this study, we sampled the contaminated and uncontaminated soils in the area surrounding crude oil pumping wells at the Changqing oilfield. The Real-time Quantitative PCR (qPCR) was used to detect the abundance of phoD gene. The diversities and compositions of phoD-haboring microbial communities were illustrated via Illumina high-throughput sequencing. Coincident soil chemical properties (soil water content (SWC), total petroleum hydrocarbons (TPHs), total nitrogen (TN), soil organic carbon (SOC), total phosphorus (TP), nitrate (NO3--N), ammonium (NH4+-N), dissolved organic carbon (DOC), available phosphorus (AP)) and ALP activities were also quantified. Results: We observed that petroleum contamination markedly decreased the abundance, richness, and diversity of the phoD-harboring bacterial community but greatly enhanced the relative abundance of phoD-harboring Actinomycetia, Thermoleophilia, and Rubrobacteria (p < 0.05). The relative abundances of phoD-harboring Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria showed an increasing tendency and then decreased as the oil contamination concentration increased (p < 0.05). The soil water, nutrient content [carbon (C), nitrogen (N), and phosphorus], and nutrient ratio were the crucial parameters influencing the phoD-harboring bacterial community responding to oil contamination. The activity of ALP was associated positively and negatively with the relative abundance of Betaproteobacteria and Rubrobacteria, respectively. Discussion: Overall, the oil pollution stress altered the abundance, richness and composition of the active phoD-harboring functional microbial community. A significant decline in ALP activity in the oil-contaminated soils was likely caused by reduced abundance and changes in the composition of the phoD-harboring bacterial community, which were strongly dependent on the available N and P contents.
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WOS关键词LONG-TERM NITROGEN ; MICROBIAL COMMUNITIES ; GENE DIVERSITY ; PROFILES ; ARCHAEAL
WOS研究方向Microbiology
语种英语
WOS记录号WOS:001671594600001
出版者FRONTIERS MEDIA SA
源URL[http://ir.igsnrr.ac.cn/handle/311030/221045]  
专题生态系统网络观测与模拟院重点实验室_外文论文
通讯作者Li, Xingchun
作者单位1.Northeast Forestry Univ, Inst Carbon Neutral, Sch Ecol,Minist Educ, Key Lab Sustainable Forest Ecosyst Management, Harbin, Peoples R China;
2.New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand
3.Northeast Forestry Univ, Coll Life Sci, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin, Peoples R China;
4.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing, Peoples R China;
5.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China;
6.CNPC Res Inst Safety & Environm Technol, State Key Lab Petr Pollut Control, Beijing, Peoples R China;
推荐引用方式
GB/T 7714		 Li, Dandan,Du, Xianyuan,Li, Xingchun,et al. Main controls on phoD-harboring bacterial community abundance, composition, and activity from oil-contaminated soils at the Changqing oilfield, Northwest China[J]. FRONTIERS IN MICROBIOLOGY,2026,16:1732638.
APA Li, Dandan.,Du, Xianyuan.,Li, Xingchun.,Zhang, Xinyu.,Zheng, Jin.,...&Wang, Qingwei.(2026).Main controls on phoD-harboring bacterial community abundance, composition, and activity from oil-contaminated soils at the Changqing oilfield, Northwest China.FRONTIERS IN MICROBIOLOGY,16,1732638.
MLA Li, Dandan,et al."Main controls on phoD-harboring bacterial community abundance, composition, and activity from oil-contaminated soils at the Changqing oilfield, Northwest China".FRONTIERS IN MICROBIOLOGY 16(2026):1732638.

入库方式： OAI收割

来源：地理科学与资源研究所

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