This article introduces the design and performance of the data acquisition system used in an omnidirectional gamma-ray positioning system, along with a new method used in this system to obtain the position of radiation sources in a large field. This data acquisition system has various built-in interfaces collecting, in real time, information from the radiation detector, the video camera and the GPS positioning module. Experiments show that the data acquisition system is capable of carrying out the proposed quantitative analysis to derive the position of radioactive sources, which also satisfies the requirements of high stability and reliability.