Scheelite is one of the main ore minerals in tungsten deposits, and its geochemical features can be used to examine and constrain tungsten mineralization. The Yangla deposit is one of the most important Cu-polymetallic skarn-porphyry deposits in the “Sanjiang” polymetallic metallogenic domain, Southwest China, hosting 150 Mt of Cu ores with economic concentrations of Pb, Zn, and Sb. The newly discovered tungsten mineralization in the Yangla deposit remains poorly understood. Hence, in order to ascertain the timing of tungsten mineralization, investigate its genetic relationship with magmatism and ore genesis, and elucidate the source, nature, and evolution of the ore-forming fluids, we performed a combined study of geochronology, in-situ trace elements, fluid inclusions, H-O isotopes, and in-situ Sr isotopic compositions of scheelite. In-situ U-Pb dating of scheelite showed that the timing of tungsten mineralization was 30 Ma. This mineralization age is significantly later than the published Cu mineralization age (∼230 Ma), skarn (∼231 Ma), and granitoid emplacement age (∼230 Ma) in the Yangla ore district. In-situ trace element analyses revealed that scheelite is depleted in Mo, Mn, and Ta and slight enriched in Sr, Y, REEs, and Au. It is also characterized by HREE-depleted chondrite-normalized patterns with strongly positive Eu anomalies. Our data reveal that 3Ca²⁺ = (2REE)³⁺+□Ca (site vacancy) is the main substitution mechanism that controls REEs in scheelite. The low concentrations of Mo, negative Ce anomalies, and positive Eu anomalies of scheelite indicate a reduced condition. Preliminary REEs features indicate that the genesis of scheelite may be related to vein-type W (Au) deposits. The homogenization temperatures for fluid inclusions present a strongly decreasing trend (328 to 173 ℃), indicate that cooling is the predominant mechanism for the formation of scheelite. The δD and δ¹⁸O_fluid values from fluid inclusions of scheelite vary broadly from −161.70 ‰ to −133.30 ‰ and from 6.32 ‰ to 9.80 ‰, respectively, indicating a mixture of magmatic and meteoric water, with possible involvement of magma degassing and fluid–rock interaction. The initial ⁸⁷Sr/⁸⁶Sr ratios of scheelites (0.71931– 0.72117) are significantly higher than those of the granodiorite (0.71149 – 0.71990) and basalt (0.70562 – 0.70995) in the Yangla ore district, indicating that the Oligocene magmatism and Devonian marble with radiogenic Sr isotopic compositions contributed to tungsten mineralization. The fluid-rock interaction between the ore-forming fluids derived from the concealed granitoids and the Devonian wall rocks may play an important role in tungsten mineralization and provide significant Ca for scheelite precipitation. Thus, the tungsten mineralization should be associated with Oligocene magmatism, ruling out the genetic relationship with Late Triassic magmatism and being significantly different from the skarn-porphyry Cu-Pb-Zn mineralization at Yangla.