RNAi-mediated gene knockdown and anti-Vibrio parahaemolyticus effects in Penaeus vannamei using mPEG-PEI-c-SWNTs siRNA system
文献类型：期刊论文
| 作者 | Men, Jia L.3; Xiang, Jian H.2; Zhou, Hai L.1,3 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2025-09-30 |
| 卷号 | 608页码:14 |
| 关键词 | Carbon nanotubes siRNA delivery Gene knockdown |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2025.742742 |
| 通讯作者 | Zhou, Hai L.(zhouhl@hainanu.edu.cn) |
| 英文摘要 | Efficient delivery systems for small interfering RNA (siRNA) are essential for advancing RNA interference (RNAi) technology in aquaculture. Here, we developed and optimized a novel siRNA delivery system based on polyethylene glycol (PEG) and polyethyleneimine (PEI) functionalized carboxylic single-walled carbon nanotubes (mPEG-PEI-c-SWNTs) specifically for the aquaculture-important shrimp P. vannamei. The PEI binds siRNA and releases it in vivo, while PEG prolongs its circulation time in vivo, and SWNTs protect it from nuclease degradation while aiding in cell membrane penetration. Using this system to deliver siRNA targeting paired box protein 6 gene of P. vannamei (LvPax6) showed the effectiveness and safety of siRNA delivery, achieving significant gene knockdown. Furthermore, by targeting the aminopeptidase N1 protein of P. vannamei (LvAPN1), which serves as the receptor for the V. parahaemolyticus that causes acute hepatopancreatic necrosis disease (AHPND) (VPAHPND) toxin, the mPEG-PEI-c-SWNTs-siLvAPN1 effectively enhances resistance to VPAHPND infection. This work highlights the potential of mPEG-PEI-c-SWNTs to address current siRNA delivery challenges in aquatic organisms and provides a promising strategy for RNAi applications in aquaculture. |
| WOS关键词 | CARBON NANOTUBES ; SHRIMP ; INTERFERENCE ; NANOMATERIALS ; ACCUMULATION ; NANOPARTICLE ; AQUACULTURE ; INFECTIONS ; RESISTANCE |
| 资助项目 | National Natural Science Foundation of China[42377273] ; Hainan Province Science and Technology Special Fund[ZDYF2022SHFZ321] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001501474200001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202163]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Zhou, Hai L. |
| 作者单位 | 1.Hainan Univ, State Key Lab South China Sea Marine Resource Util, Haikou 570228, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Hainan Univ, Collaborat Innovat Ctr Life & Hlth, Sch Life & Hlth Sci, Hainan Prov Key Lab One Hlth, Haikou 570228, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Men, Jia L.,Xiang, Jian H.,Zhou, Hai L.. RNAi-mediated gene knockdown and anti-Vibrio parahaemolyticus effects in Penaeus vannamei using mPEG-PEI-c-SWNTs siRNA system[J]. AQUACULTURE,2025,608:14. |
| APA | Men, Jia L.,Xiang, Jian H.,&Zhou, Hai L..(2025).RNAi-mediated gene knockdown and anti-Vibrio parahaemolyticus effects in Penaeus vannamei using mPEG-PEI-c-SWNTs siRNA system.AQUACULTURE,608,14. |
| MLA | Men, Jia L.,et al."RNAi-mediated gene knockdown and anti-Vibrio parahaemolyticus effects in Penaeus vannamei using mPEG-PEI-c-SWNTs siRNA system".AQUACULTURE 608(2025):14. |
入库方式： OAI收割
来源：海洋研究所
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